ArticleThe Journal of cell biology1985
Development of fetal rat intestine in organ and monolayer culture.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 80 citations in OpenAlex.
- Intestinal epithelial cells in vitro.Stem cells and development · 2010Review
- Establishment of conditionally immortalized epithelial cell lines from the intestinal tissue of adult normal and transgenic mice.American journal of physiology. Gastrointestinal and liver physiology · 2009Review
- Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures.BMC cell biology · 2005Article
- Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases.Molecular and cellular biology · 2004Article
- Regulation and expression of multidrug resistance (MDR) transcripts in the intestinal epithelium.British journal of cancer · 1999Article
- Contraction of collagen gels by intestinal epithelial cells depends on microfilament function.Digestive diseases and sciences · 1993Article
- Establishment of conditionally immortalized epithelial cell lines from both colon and small intestine of adult H-2Kb-tsA58 transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Clonal analysis of sucrase-isomaltase expression in the human colon adenocarcinoma Caco-2 cells.The Biochemical journal · 1991Article
- Explant organ culture: a review.Cytotechnology · 1991Review
- Polarized intestinal hybrid cell lines derived from primary culture: establishment and characterization.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Phenotype alteration in colon carcinoma cells: effect of in vivo passage?In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1991Article
- In vitro model of attachment of Giardia intestinalis trophozoites to IEC-6 cells, an intestinal cell line.Antimicrobial agents and chemotherapy · 1991Article
- Glycosylation in intestinal epithelium.International review of cytology · 1991Review
- Myogenesis and histogenesis of skeletal muscle on flexible membranes in vitro.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Article
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- Transfection of fetal rat intestinal epithelial cells by viral oncogenes: establishment and characterization of the E1A-immortalized SLC-11 cell line.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine.Molecular and cellular biology · 1988Article
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Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Maturation and differentiation of intestinal epithelial cells was demonstrated in segments of fetal rat small intestine, maintained for more than a month in suspension organ culture, by ultrastructural, biochemical, and immunological criteria. Over a 5-7 d period, fragments of fetal intestine evolved into globular structures covered with a single columnar epithelium ultrastructurally similar to suckling villus cells. Loose mesenchymal cells, cellular debris, and collagen were present inside the structures. After 6 d in culture, goblet cells, not present in the fetal intestine at day 18, were numerous and well developed. Intestinal endocrine cells were also observed. Immunofluorescence studies employing monoclonal antibodies specific for villus and crypt cells in vivo, and various enzyme assays, have demonstrated a level of differentiation and maturation of the cultured epithelial cells similar but not identical to that of suckling intestinal mucosa in vivo. Crypts and crypt cell markers were not observed in the the cultures. Addition of glucocorticoids to the culture medium resulted in the induction of sucrase-isomaltase but failed to promote most of the functional changes characteristic of the intestinal epithelium at weaning in vivo. Epithelial cells were identified in explants derived from the organ cultures by their specific expression of intestinal cytokeratin. Differentiation-specific markers, present in the epithelial cells in primary cultures, were lost upon selection and subculturing of pure epithelial cell populations. These results suggest a requirement for mesenchymal and/or extracellular matrix components in the maintenance of the differentiated state of the epithelial cells. The fetal intestinal organ cultures described here present significant advantages over traditional organ and monolayer culture techniques for the study of the cellular and molecular interactions involved in the development and differentiation of the intestinal epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.