ArticlePlant biotechnology journal2025
A modulatory role of CG methylation on gene expression in soybean implicates its potential utility in breeding.
Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Genome-Wide DNA Methylation Analysis of the Toxicological Responses to Foliar Cerium Application in Soybean.Toxics · 2026Article
- Breeding Climate-Resilient Soybeans for 2050 and Beyond: Leveraging Novel Technologies to Mitigate Yield Stagnation and Climate Change Impacts.Plants (Basel, Switzerland) · 2026Review
- Genetic and epigenetic mechanisms underlying cadmium (Cd) stress responses in soybean cultivars.Plant cell reports · 2026Article
- The mirage of DNA methylation in transcriptional regulation of plants.The plant genome · 2026Review
- Structure and autoinhibitory regulation of MET1 in the maintenance of plant CG methylation.The Plant cell · 2025Article
- Review
- Functional Genomics: From Soybean to Legume.International journal of molecular sciences · 2025Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Cytosine methylation (mCG) is an important heritable epigenetic modification, yet its functions remain to be fully defined in important crops. This study investigates mCG in soybean following the loss-of-function mutation of two GmMET1 genes. We generated knockout mutants of GmMET1s by CRISPR-Cas9 and conducted comprehensive methylome and transcriptome analyses. Our findings unravel the functional redundancy of the two GmMET1s, with GmMET1b being more critically involved in maintaining mCG levels, and complete knockout of both copies is lethal. We establish that genome-wide mCG levels scale with aggregated expression of GmMET1s. We identify a set of mCG-regulated genes whose expression levels were quantitatively modulated by upstream, body, or downstream mCG. Moreover, we find genes that were negatively regulated by upstream or body mCG are enriched in specific biological processes such as that of jasmonic acid metabolism. Notably, >80% of the differentially methylated genes (DMGs) in the mutants also exist as DMGs in natural soybean populations. Phenotypically, mutants that are heterozygous for GmMET1a and homozygous for GmMET1b knockouts (GmMET1a
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Registered trials
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