Evidence map›Paper›PMID 39887619›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Multi-Step Assembly of an RNA-Liposome Nanoparticle Formulation Revealed by Real-Time, Single-Particle Quantitative Imaging.

Michael C Chung, Hector R Mendez-Gomez, Dhruvkumar Soni, Reagan McGinley, Alen Zacharia, Jewel Ashbrook, Brian Stover, Adam J Grippin, Elias J Sayour, Juan Guan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Michael C ChungDivision of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, TX, 78712, USA.
Hector R Mendez-GomezDepartment of Neurosurgery, Preston A. Wells, Jr. Center for Brain Tumor Therapy, McKnight Brain Institute, University of Florida Lillian S. Wells, Gainesville, FL, 32610, USA.
Dhruvkumar SoniDepartment of Neurosurgery, Preston A. Wells, Jr. Center for Brain Tumor Therapy, McKnight Brain Institute, University of Florida Lillian S. Wells, Gainesville, FL, 32610, USA.
Reagan McGinleyDepartment of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32603, USA.
Alen ZachariaDepartment of Physics, University of Florida, Gainesville, FL, 32611, USA.ORCID https://orcid.org/0009-0003-5834-8755
Jewel AshbrookMiddlebury College Department of Physics, McCardell Bicentennial Hall, Middlebury, VT, 05753, USA.
Brian StoverDepartment of Pediatrics, Division of Pediatric Hematology Oncology, University of Florida, Gainesville, FL, 32610, USA.
Adam J GrippinMD Anderson Cancer Center, Division of Radiation Oncology, University of Texas, Houston, TX, 77030, USA.
Elias J SayourDepartment of Neurosurgery, Preston A. Wells, Jr. Center for Brain Tumor Therapy, McKnight Brain Institute, University of Florida Lillian S. Wells, Gainesville, FL, 32610, USA.
Juan GuanDivision of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, TX, 78712, USA.ORCID https://orcid.org/0000-0002-4088-8126

Funding

Overcoming the blood-brain barrier with nanoparticle vaccines against gliomasR37CA251978 · NCI · UNIVERSITY OF FLORIDA · PI Elias Sayour · 2021 to 2026
$3.4M
Interdisciplinary Translational Pre/Postdoctoral Program in Cancer NanotechnologyT32CA196561 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI Gang Bao, Konstantin V Sokolov · 2015 to 2026
$3.0M
Overcoming metastatic spread of osteosarcoma with RNA loaded nanoparticlesR01CA266857 · NCI · UNIVERSITY OF FLORIDA · PI SAYOUR, ELIAS · 2022 to 2025
$2.9M
Mechanisms of Assembly and Functional Regulation in Non-canonical Biomolecular CondensatesR35GM146877 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Juan Guan · 2022 to 2026
$2.0M
FDA HHS R01 FD007268FDA HHS R01FD007268Florida Department of Health 20B11Florida Department of Health 20L07NCI NIH HHS R01 CA266857NCI NIH HHS R01CA266857NCI NIH HHS R37 CA251978NCI NIH HHS R37CA251978NCI NIH HHS T32 CA196561NIGMS NIH HHS R35 GM146877NIGMS NIH HHS R35GM146877
6 · The paper itself

Abstract

Self-assembly plays a critical role in nanoparticle-based applications. However, it remains challenging to monitor the self-assembly of multi-component nanomaterials at a single-particle level, in real-time, with high throughput, and in a model-independent manner. Here, multi-color fluorescence microscopy is applied to track the assembly of both liposomes and mRNA simultaneously in clinical mRNA-based cancer immunotherapy. Imaging reveals that the assembly occurs in discrete steps: initially, RNA adsorbs onto the liposomes; then, the RNA-coated liposomes cluster into heterogeneous structures ranging from sub-micrometer to tens of micrometers. The clustering process is consistent with a Smoluchowski model with a Brownian diffusion kernel. The transition between the two steps of assembly is determined by the orientation of RNA-mediated interactions. Given the facile application of this approach and the ubiquity of the components studied, the imaging and analysis in this work are readily applied to monitor multi-component assembly of diverse nanomaterials.

Indexed as

LiposomesNanoparticlesRNAHumansMicroscopy, FluorescenceRNA, MessengerLiposomesRNARNA, Messengercancer‐therapydrug‐deliveryimagingnanomaterialsnanoparticlesquantitative imagingself‐assembly

Identifiers

PMID39887619
PMCPMC11948016

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.