Evidence map›Paper›PMID 39887113›Full record

ArticleParasite (Paris, France)2025

Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi.

Xinan Meng, Yonglin Ou, Wen Jiang, Yaqiong Guo, Lihua Xiao, Yaoyu Feng, Na Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Investigation of Infection ofVeterinary sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xinan MengState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Yonglin OuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Wen JiangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Yaqiong GuoState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China - Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Lihua XiaoState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China - Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Yaoyu FengState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China - Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Na LiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, South China Agricultural University, Guangzhou 510642, China - Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Funding

111 Center D20008Double First-Class Discipline Promotion Project 2023B10564003National Natural Science Foundation of China 32030109National Natural Science Foundation of China U21A20258
6 · The paper itself

Abstract

In addition to the ribosomal internal transcribed spacer (ITS) locus, four loci (MS1, MS3, MS4, and MS7) have been identified to develop multilocus sequence typing tools for high-resolution genotyping of Enterocytozoon bieneusi in previous studies. However, the use of only five loci was insufficient for population genetic analysis of E. bieneusi from diverse hosts. In this study, comparison of a clinical genome sequence (C44566) with the whole genome sequence of an E. bieneusi isolate (H348) in GenBank led to the selection of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci. Further analysis of the two loci with 156 E. bieneusi-positive samples showed high sequence polymorphisms in ITS Groups 1-6 and 10. Altogether, 30 and 23 sequence types were identified at hp1 and tub1, respectively. Genotyping based on the two loci confirmed the lack of genetic differentiation between Group 1 and Group 2 genotypes, as previously reported. Moreover, the genotypes in Groups 4 and 5 are more divergent from other genotypes within Groups 1-10. However, isolates in Group 11 and 12 could not be amplified at the hp1 and tub1 loci, supporting the previous conclusion of genetic uniqueness of the two genotype groups. The identified genetic markers and generated data could be used to develop a multilocus sequence typing tool for high-resolution genotyping of E. bieneusi, which would also have implications for understanding the taxonomy of Enterocytozoon spp., the public health significance of E. bieneusi in animals, and sources of E. bieneusi infections in humans.

Indexed as

EnterocytozoonGenotypeFungal ProteinsGenetic LociGenome, FungalHumansMicrosporidiosisPhylogenyPolymerase Chain ReactionTubulinFungal ProteinsTubulinEnterocytozoon bieneusiGenetic diversityGenetic markerMultilocus sequence typing

Identifiers

PMID39887113
PMCPMC11784105

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.