Evidence map›Paper›PMID 39886789›Full record

ArticleCombinatorial chemistry & high throughput screening2026

Forsythiaside A Ameliorates Inflammation by Regulating the Autophagy in Methotrexate-induced Intestinal Mucositis.

Wuying Lang, Jiayi Zhang, Xuejun Xiao, Min Cheng, Xin Zheng, Haizhou Gong, Ihsan Ali, Yongping Zhao, Feng Jia, Zhe Wang and 3 more

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Article in Combinatorial chemistry & high throughput screening, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wuying LangCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Jiayi ZhangCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Xuejun XiaoDepartment of Medical Oncology, Guangshan County People's Hospital, Xinyang, China.
Min ChengCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Xin ZhengCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Haizhou GongCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Ihsan AliCollege of Veterinary Science Faculty of Animal Husbandry and Veterinary Science, the University of Agriculture Peshawar, Pakistan.
Yongping ZhaoCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Feng JiaCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Zhe WangCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Jing WangCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Wei LiCollege of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.
Haihua ZhangHebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.

Funding

Academician Special Project of Shangluo University 20YSZX04Agricultural Industry System of Hebei Province HBCT2023210202College Students' Innovative Entrepreneurial Training Plan Program S202211396071Department of Science and Technology of Shaanxi Province 2024JC-YBQN-0178Doctoral Startup Foundation of Shangluo University of Science and Technology 20SKY011Education Department of Shaanxi Provincial Government 22JHQ068Innovation Capability Support of Shaanxi 2021ZDLSF04-05Key Research and Development Plan of Hebei Province 22326613DNational Key R&D Projects 2023YFD130200Project of Shaanxi Provincial Science and Technology Department 2024ZC-YYDP-89Science and Technology Planning Project of Shangluo City 2023-N-0036Science and Technology Plan Project of Shangluo 2022-Y-0051Shaanxi Provincial Science and Technology Department Key R&D Program Projects 2024NC-YBXM-263Shaanxi Provincial Science and Technology Innovation Guidance Special Program 2023QYPY2-01Shaanxi Science and Technology Innovation Team 2022TD-56Third Batch of the Top Talent Project for Youths of Hebei Province, the Natural Science Basic Research Plan in Shaanxi Province of China 2023-JC-QN-0270
6 · The paper itself

Abstract

backgroundMethotrexate (MTX) effectively eliminates cancerous cells but can also cause inflammation intestinal, known as mucositis. Forsythiaside A (FTA) from Forsythia suspensa has shown promise in relieving mucositis by targeting the NLRP3 pathways. Since NLRP3 inflammasome activation is negatively regulated by autophagy, this study explores how FTAmediated autophagy affects NLRP3 inflammasome in treating MTX-induced intestinal inflammation.

methodsIntestinal mucositis was induced in rats with MTX. FTA's impact was assessed using HE staining and ELISA. The mechanism was studied using immunofluorescence, western blot, and ELISA.

resultsFTA treatment resulted in reduced levels of D-lactic acid and diamine oxidase (DAO) in MTX-treated rats. Western blot and immunofluorescence analyses revealed up-regulation of Beclin- 1 and LC3II/I, accumulation of LC3, and down-regulation of p62 expression levels in MTXtreated rats following 40 or 80 mg/kg FTA intervention. However, when the autophagy inhibitor 3-MA was used, the intestinal pathology was exacerbated, the inflammatory scores increased, and serum levels of TNF-α, IL-1β, and IL-18 were elevated. Western blotting indicated decreased LC3II/I expression, while NLRP3, cleaved caspase 1, and cleaved IL-1β expressions were upregulated.

conclusionThese findings suggested that FTA alleviated MTX-treated intestinal mucositis by activating autophagy, which in turn inhibits the NLRP3 inflammasome.

Indexed as

AutophagyGlycosidesInflammationMethotrexateMucositisAnimalsMaleNLR Family, Pyrin Domain-Containing 3 ProteinRatsRats, Sprague-DawleyforsythiasideGlycosidesMethotrexateNLR Family, Pyrin Domain-Containing 3 ProteinautophagyForsythiaside Aintestinal mucositisLC3II/ImethotrexateNLRP3 inflammasome

Identifiers

PMID39886789

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.