Evidence map›Paper›PMID 39886022›Full record

ArticlePeerJ2025

A novel ddPCR™ assay for eDNA detection and quantification of Greater Amberjack

P Joana Dias, Ryan Lehman, Bryan L Huerta-Beltrán, Ana Wheeler, Crystal L Hightower, Jessica Heise, Theodore Switzer, Clint Rhode, J Marcus Drymon, Lynne Stokes and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

P Joana DiasSchool of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, United States.
Ryan LehmanSchool of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, United States.
Bryan L Huerta-BeltránSchool of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, United States.ORCID 0000-0003-1873-9826
Ana WheelerMississippi-Alabama Sea Grant Consortium, Ocean Springs, MS, United States.ORCID 0000-0003-3446-2943
Crystal L HightowerStokes School of Marine and Environmental Sciences, University of South Alabama, Mobile, AL, United States.ORCID 0000-0002-9197-4809
Jessica HeiseFish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St Petersburg, FL, United States.
Theodore SwitzerFish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, St Petersburg, FL, United States.
Clint RhodeDepartment of Genetics, Stellenbosch University, Stellenbosch, South Africa.ORCID 0000-0001-7969-7282
J Marcus DrymonMississippi-Alabama Sea Grant Consortium, Ocean Springs, MS, United States.ORCID 0000-0002-2104-004X
Lynne StokesDepartment of Statistical Science, Southern Methodist University, Dallas, TX, United States.
Mark A AlbinsStokes School of Marine and Environmental Sciences, University of South Alabama, Mobile, AL, United States.ORCID 0000-0002-6020-1205
Sean PowersStokes School of Marine and Environmental Sciences, University of South Alabama, Mobile, AL, United States.
Nicole PhillipsSchool of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, United States.

Funding

Training and Mentoring Core P20GM103476 · NIGMS · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI MICHAEL R GARRETT · 2012 to 2026
$60.2M
NIGMS NIH HHS P20 GM103476
6 · The paper itself

Abstract

Background: Four Methods: We developed and validated a novel tetraplex ddPCR™ assay able to detect and distinguish between Results: We demonstrated that with eDNA contamination controls and best practice protocols, it is viable to conduct eDNA research as part of a fisheries survey cruise. eDNA sampling was completed in less time than camera gears (15 min Discussion: Incorporating eDNA in fisheries surveys would not require additional days at sea and could improve precision in fish detection and abundance. eDNA can be a valuable complement to camera gears deployed in geographic areas or seasons with poor visibility conditions, where fish may be present but cannot be confidently identified to the species level. The high correlation obtained between ROV and eDNA data collected between gear deployments adds to a growing number of studies demonstrating the potential of eDNA as an indicator of abundance for fisheries stock assessments. Time-series data from a carefully designed eDNA survey, that estimates relative abundance, could be used as an index of relative abundance for the

Indexed as

DNA, EnvironmentalEnvironmental MonitoringFisheriesPolymerase Chain ReactionAnimalsAtlantic OceanGulf of AmericaSeawaterUnited StatesDNA, EnvironmentalEnvironmental DNASeriola fasciataSeriola rivolianaSeriola zonataTetraplex droplet digital™ PCR

Identifiers

PMID39886022
PMCPMC11781265

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.