Evidence map›Paper›PMID 39885598›Full record

ArticleVeterinary research2025

Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.

Koji Nishi, Satoshi Gondaira, Yuki Hirano, Masahide Ohashi, Ayano Sato, Kazuya Matsuda, Tomohito Iwasaki, Takuya Kanda, Ryoko Uemura, Hidetoshi Higuchi

Abstract read
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Comprehensive Characterization ofVeterinary sciences · 2025
    Article
  4. Microorganisms · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Koji NishiAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Satoshi GondairaAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan. s-gondaira@rakuno.ac.jp.ORCID http://orcid.org/0000-0001-9745-7792
Yuki HiranoAnimal Research Center, Agricultural Research Department, Hokkaido Research Organization, Shintoku, Hokkaido, Japan.
Masahide OhashiAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Ayano SatoLarge Animal Clinical Science, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Kazuya MatsudaDepartment of Veterinary Pathology, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.
Tomohito IwasakiDepartment of Food Science and Human Wellness, College of Agriculture, Food and Environment Science, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Takuya KandaFood Safety Laboratory, Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan.
Ryoko UemuraAnimal Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Hidetoshi HiguchiAnimal Health Unit, Department of Veterinary Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan. higuchi@rakuno.ac.jp.

Funding

Japan Society for the Promotion of Science London 23K05574
6 · The paper itself

Abstract

Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 10

Indexed as

ActinomycetaceaeBiofilmsCattle DiseasesMycoplasma bovisPneumonia, MycoplasmaAnimalsCattleCoculture TechniquesCoinfectionAntibioticsantimicrobial agentsbovine respiratory diseaseextracellular matrixtrachea

Identifiers

PMID39885598
PMCPMC11783866

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.