Evidence map›Paper›PMID 39885446›Full record

ArticleBMC veterinary research2025

Development and application of reverse transcriptase droplet digital PCR technology for sensitive detection of BVDV-1 and BTV in bovine semen.

Zhichao Yu, Linjun Chen, Qiang Cui, Han Yan, Junyan Li, Xiaoping Luo, Yingying Li, Xianghong Ju, Yanhong Yong, Namula Zhao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhichao YuDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
Linjun ChenTechnology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Qiang CuiTechnology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Han YanTechnology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China.
Junyan LiKey Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, Inner Mongolia, China.
Xiaoping LuoKey Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, Inner Mongolia, China.
Yingying LiAnimal and Plant Quarantine and Animal Disease Prevention and Control Center, Baotou, 014016, Inner Mongolia, China.
Xianghong JuDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
Yanhong YongDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
Namula ZhaoDepartment of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, China.
Zhiguo ZhaoTechnology Center, Hohhot Customs District, Hohhot, 010020, Inner Mongolia, China. zhaozhiguo303@163.com.

Funding

Guangdong provincial key research and development programme 2022B0202090003Inner Mongolia Autonomous Region Major Science and Technology Special Project 2020ZD0006Key research and development and results transformation plan of Inner Mongolia Autonomous Region 2023YFDZ0048Key Technology Project of Inner Mongolia Science and Technology Department 2021GG0171Science and technology project of General Administration of Customs 2023HK012the scientific research start-up funds of Guangdong Ocean University R20041
6 · The paper itself

Abstract

backgroundBovine viral diarrhoea virus genotype 1 (BVDV-1) and bluetongue virus (BTV) are potent viral pathogens that may be transmitted through semen, resulting in the spread of diseases via artificial insemination. Thus, establishing an early detection method for BVDV-1 and BTV infection is important for the trading of semen. In this study, we developed two RT‒ddPCR methods to detect BVDV-1 and BTV, and each method was evaluated for repeatability, limit of detection and specificity. The sensitivity of these methods was compared with that of RT‒qPCR (WOAH) by analysing clinical samples.

resultsThe RT‒ddPCR results revealed that both methods exhibited good repeatability at low concentrations, with detection limits of 1.05 copies/µL and 0.662 copies/µL per reaction for BVDV-1 and BTV, respectively; additionally, both methods exhibited high specificity and did not exhibit cross-reaction with other important semen-transmitted pathogens. Eighty bovine semen samples and twenty mixed semen samples were tested. The results revealed that the positivity rates of BVDV-1 and BTV RT‒ddPCR (25% and 23%, respectively) were greater than those of RT‒qPCR (19% and 18%, respectively).

conclusionsRT‒ddPCR was highly sensitive for detecting low concentrations of BVDV-1 and BTV in clinical samples and could be a good supplement for qPCR testing.

Indexed as

BluetongueBluetongue virusBovine Virus Diarrhea-Mucosal DiseaseDiarrhea Virus 1, Bovine ViralReverse Transcriptase Polymerase Chain ReactionSemenAnimalsCattleMaleReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityBluetongue virusBovine semenBovine viral diarrhoea virus genotype 1Droplet digital PCR

Identifiers

PMID39885446
PMCPMC11783913

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.