Evidence map›Paper›PMID 39885036›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Sample Preparation of Caenorhabditis elegans for GC-MS-Based Metabolomics in Toxicity Assessment.

Jesus G Arellano Spadaro, Marjolein Wildwater, Henrie Korthout, Hye Kyong Kim

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jesus G Arellano SpadaroVivaltes BV, Bunnik, The Netherlands.
Marjolein WildwaterVivaltes BV, Bunnik, The Netherlands.
Henrie KorthoutFytagoras BV, Leiden, The Netherlands.
Hye Kyong KimFytagoras BV, Leiden, The Netherlands. h.k.kim@biology.leidenuniv.nl.ORCID 0000-0002-6956-7449

Funding

National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C012103/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C012202/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C017101/1
6 · The paper itself

Abstract

The nematode Caenorhabditis elegans, widely recognized as a model organism due to its ease of breeding and well-characterized genomes, boasts complete digestive, reproductive, and endocrine systems, as well as conserved signaling pathways shared with mammals. It has become an invaluable resource for metabolomics research, particularly in examining responses to chemical or environmental factors and toxicity assessments. In this article, we provide detailed, step-by-step protocols for cultivating C. elegans and conducting metabolomics analyses, specifically focusing on sample preparation for GC-MS analysis in response to toxic compound treatments. We highlight the critical choice of extraction solvent, introducing two representative systems for extracting metabolites from C. elegans.

Indexed as

Caenorhabditis elegansGas Chromatography-Mass SpectrometryMetabolomicsToxicity TestsAnimalsMetabolomeCaenorhabditis elegansExtractionExtraction solventGC-MS analysisMetabolomicsToxicity assessment

Identifiers

PMID39885036

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.