Evidence map›Paper›PMID 39880862›Full record

ArticleScientific reports2025

Modulation of biological activities in adipose derived stem cells by histone deacetylation.

Sallam Abdallah, Mouna Tabebi, Sawsan Qanadilo, Neserin Ali, Jing Wang, Pádraig D'Arcy, Wen Zhong, Folke Sjoberg, Moustafa Elmasry, Ahmed El-Serafi

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sallam AbdallahThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Mouna TabebiThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Sawsan QanadiloDepartment of Biological Sciences, The University of Jordan, Amman, Jordan.
Neserin AliDepartment of Clinical Sciences, Lund University, Lund, Sweden.
Jing WangThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Pádraig D'ArcyThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Wen ZhongThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Folke SjobergThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Moustafa ElmasryThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden.
Ahmed El-SerafiThe Department of Biomedical and Clinical Sciences (BKV), Linköping University, Linköping, Sweden. ahmed.elserafy@liu.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Difficult-to-heal wounds management accounts for about 4% of healthcare costs, highlighting the need for innovative solutions. Extracellular signals drive cell proliferation during tissue regeneration, while epigenetic mechanisms regulate stem cell homeostasis, differentiation, and skin repair. Exploring epigenetic regulation in adipose-derived stem cells (ADSCs) holds promise for improving skin injury treatments. We investigated the effects of histone deacetylase inhibitor (SAHA) on ADSCs to better understand its cellular and molecular impacts. ADSCs were treated with SAHA for 72 h, showing no change in cell viability at the studied concentrations. However, the expression of histone deacetylase decreased at 1000 nM, while the cell proliferation marker Ki-67 increased after SAHA treatment, as confirmed by immunofluorescence. CCND1 gene expression increased, whereas protein expression of the proliferating cell nuclear antigen (PCNA) decreased. Cell cycle analysis showed an increase in G2 phase in SAHA-treated cells. Microarray analysis revealed 74 upregulated and 40 downregulated differentially expressed genes, including upregulation of P53 targets, CDKN1A and MDM2. Proteomic analysis identified 631 upregulated and 823 downregulated proteins compared to the vehicle. Pathway enrichment analysis showed cell cycle, ATP-dependent chromatin remodeling and DNA processes were among the affected pathways. This study suggests SAHA modulates ADSCs' biological processes, highlighting its potential for skin regeneration.

Indexed as

Adipose TissueHistone Deacetylase InhibitorsHistone DeacetylasesHistonesStem CellsAcetylationCell CycleCell DifferentiationCell ProliferationCells, CulturedCell SurvivalEpigenesis, GeneticHumansVorinostatHistone Deacetylase InhibitorsHistone DeacetylasesHistonesVorinostatAdipose derived stem cellsDifferentiationEpigeneticEpigenetic modifierHistone deacetylase inhibitorSuberoylanilide hydroxamic acidVorinostat

Identifiers

PMID39880862
PMCPMC11779964

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.