ArticleJournal of virological methods2025
Endothelial growth media components alter SARS-CoV-2 spike-directed growth kinetics.
Article in Journal of virological methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Direct SARS-CoV-2 infection of endothelial cells is challenging to study in vitro. To examine whether endothelial cell culture conditions impact the ability of SARS-CoV-2 to infect cells, we evaluated the effects of commercial cell culture media composition on SARS-CoV-2 Spike-directed viral infection. In African Green Monkey kidney epithelial cells (VeroE6), we found that commercial cell culture media (EGM2) produced inhibitory effects on recombinant vesicular stomatitis virus (rVSV-SARS-CoV-2) growth that is not seen in Dulbecco's Modified Eagle Medium (DMEM). We identified Lonza's GA-1000 supplement as a potential SARS-CoV-2 inhibitor. We then compared how titrations of GA-1000 influenced rVSV-SARS-CoV-2 and SARS-CoV-2 infection. The determined IC50 of GA-1000 is observed at a 1:450 dilution, about 2-fold higher concentration than the typical cell culture concentration, against rVSV-SARS-CoV-2. In contrast, the inhibition against SARS-CoV-2 infection is modest. This work highlights the importance of cell culture medium selection for in vitro models of infections and demonstrates a method to characterize and ameliorate media formulations for infection studies.
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