Evidence map›Paper›PMID 39879084›Full record

ArticleBioengineered2025

Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing in

Hyerang Eom, Yeon-Jae Choi, Rutuja Nandre, Minseek Kim, Youn-Lee Oh, Sinil Kim, Takehito Nakazawa, Yoichi Honda, Hyeon-Su Ro

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Gene Editing inJournal of fungi (Basel, Switzerland) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hyerang EomDepartment of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Yeon-Jae ChoiDepartment of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Rutuja NandreDepartment of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Minseek KimMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Republic of Korea.
Youn-Lee OhMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Republic of Korea.
Sinil KimBiological Resources Utilization Division, National Institute of Biological Resources(NIBR), Incheon, Republic of Korea.
Takehito NakazawaLaboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Yoichi HondaLaboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Hyeon-Su RoDepartment of BioMedical Bigdata (BK21) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.ORCID 0000-0003-1128-8401

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene editing is emerging as a powerful tool for introducing novel functionalities in mushrooms. While CRISPR/Cas9-induced double-strand breaks (DSBs) typically rely on non-homologous end joining (NHEJ) for gene disruption, precise insertion of heterologous DNA in mushrooms is less explored. Here, we evaluated the efficacy of inserting donor DNAs (8-1008 bp) with or without homologous arms at Cas9-gRNA RNP-induced DSBs. Co-transformation of donor DNAs with RNP targeting the

Indexed as

CRISPR-Cas SystemsGene EditingReishiRibonucleoproteinsRNA, Guide, CRISPR-Cas SystemsDNA Breaks, Double-StrandedDNA End-Joining RepairRibonucleoproteinsRNA, Guide, CRISPR-Cas SystemsGanodermagene editingHomology-directed repair (HDR)mushroomnon-homologous end joining (NHEJ)

Identifiers

PMID39879084
PMCPMC11781247

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.