Evidence map›Paper›PMID 39878844›Full record

ArticleAnnals of surgical oncology2025

Bright and Specific Targeting of Metastatic Lymph Nodes in Orthotopic Mouse Models of Gastric Cancer with a Fluorescent Anti-CEA Antibody.

Kristin E Cox, Siamak Amirfakhri, Thinzar M Lwin, Mojgan Hosseini, Pradipta Ghosh, Marygorret Obonyo, Robert M Hoffman, Paul J Yazaki, Michael Bouvet

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Article in Annals of surgical oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Kristin E CoxDepartment of Surgery, University of California San Diego, La Jolla, CA, USA.
Siamak AmirfakhriDepartment of Surgery, University of California San Diego, La Jolla, CA, USA.
Thinzar M LwinDepartment of Surgical Oncology, City of Hope National Medical Center, Duarte, CA, USA.
Mojgan HosseiniDepartment of Pathology, University of California San Diego, La Jolla, CA, USA.
Pradipta GhoshDepartment of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA.
Marygorret ObonyoDepartment of Medicine, University of California San Diego, La Jolla, CA, USA.
Robert M HoffmanDepartment of Surgery, University of California San Diego, La Jolla, CA, USA.
Paul J YazakiDepartment of Immunology & Theranostics, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Michael BouvetDepartment of Surgery, University of California San Diego, La Jolla, CA, USA. mbouvet@ucsd.edu.ORCID http://orcid.org/0000-0002-0086-2159

Funding

Curebound Foundation 20DG03National Cancer Institute Cancer Center Support Grant P30CA23100NIH HHS CA100768NIH HHS CA121938NIH HHS CA238042NIH HHS CA256973-01Padres Pedal the Cause Collaborative Translational Pilot Project PCT2022Padres Pedal the Cause Collaborative Translational Pilot Project PTC2021U.S. Department of Defense W81XWH-20-1-0675U.S. Department of Veterans Affairs 1 I01 BX003856-01A1U.S. Department of Veterans Affairs 1 I01 BX004494-01
6 · The paper itself

Abstract

backgroundGastric cancer poses a major diagnostic and therapeutic challenge. Improved visualization of tumor margins and lymph node metastases with tumor-specific fluorescent markers could improve outcomes.

methodsTo establish orthotopic models of gastric cancer, one million cells of the human gastric cancer cell line, MKN45, were suspended in 50 μl of equal parts PBS and Matrigel and injected into the nude mouse stomach with a 29-gauge needle. Tumors were allowed to grow for 8-12 weeks before performing imaging studies. For tumor labeling, M5A (humanized anti-CEA mAb) and IgG as a control, were conjugated with the near-infrared dye IRDye800CW. Mice were randomized to receive 50 μg of M5A-IR800 (n = 14) or 50 μg of IgG-IR800 (n = 14) intravenously and were imaged 72 hours later. Fluorescence imaging was performed using the LI-COR Pearl Imaging System.

resultsForty-two lymph nodes were collected from 28 mice, of which 59.5% were found to contain metastatic gastric cancer cells on pathologic examination. In mice that received M5A-IR800, there was a statistically significant difference in the mean fluorescence signal for cancer-positive lymph nodes at 0.431 (SE ± 0.224) compared with 0.105 (SE ± 0.009) for cancer-negative nodes (p: 0.002). For IgG-IR800, there was no significant difference in the mean fluorescence signal for cancer-positive nodes (0.057) compared with cancer-negative nodes (0.064), p-value 0.677.

conclusionsHumanized anti-CEA (M5A) antibodies conjugated to fluorescent dyes provide bright labeling of lymph nodes containing metastatic gastric cancer. This tumor-specific fluorescent antibody is a promising clinical tool for identifying lymph nodes containing metastatic gastric cancer.

Indexed as

Antibodies, MonoclonalCarcinoembryonic AntigenFluorescent DyesLymph NodesStomach NeoplasmsAnimalsCell Line, TumorDisease Models, AnimalFemaleHumansLymphatic MetastasisMiceMice, Inbred BALB CMice, NudeOptical ImagingTumor Cells, CulturedAntibodies, MonoclonalCarcinoembryonic AntigenFluorescent DyesCEAFluorescenceFluorescence-guided surgeryFluorescent antibodyGastric cancerLymph nodeNude mouse model

Identifiers

PMID39878844

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.