Evidence map›Paper›PMID 39878336›Full record

ArticleNano letters2025

Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy.

Taras Sych, André Görgens, Loïc Steiner, Gozde Gucluler, Ylva Huge, Farhood Alamdari, Markus Johansson, Firas Aljabery, Amir Sherif, Susanne Gabrielsson and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nano letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Taras SychScience for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Tomtebodavägen 23, 17165 Solna, Sweden.
André GörgensDivision of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska ATMP Center, Karolinska Institutet, 14152 Huddinge, Sweden.
Loïc SteinerDivision of Immunology and Respiratory Medicine, Department of Medicine Solna, Karolinska Institutet, 17164 405 Stockholm, Sweden.ORCID 0000-0001-6640-3513
Gozde GuclulerDivision of Immunology and Respiratory Medicine, Department of Medicine Solna, Karolinska Institutet, 17164 405 Stockholm, Sweden.
Ylva HugeDepartment of Urology in Östergötland and Department of Biomedical and Clinical Sciences, Linköping University, 58225 Linköping, Sweden.
Farhood AlamdariDepartment of Urology, Vastmanland Hospital, 72189 Västerås, Sweden.
Markus JohanssonDepartement of Surgery and Urology, County Hospital of Sundsvall-Härnösand, 85643 Sundsvall, Sweden.
Firas AljaberyDepartment of Urology in Östergötland, and Department of Biomedical and Clinical Sciences, Linköping University, 58225 Linköping, Sweden.
Amir SherifDepartment of Diagnostics and Intervention, Umeå University, 90187 Umeå, Sweden.
Susanne GabrielssonDivision of Immunology and Respiratory Medicine, Department of Medicine Solna, Karolinska Institutet, 17164 405 Stockholm, Sweden.
Samir El AndaloussiDivision of Biomolecular and Cellular Medicine, Department of Laboratory Medicine, Karolinska ATMP Center, Karolinska Institutet, 14152 Huddinge, Sweden.
Erdinc SezginScience for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Tomtebodavägen 23, 17165 Solna, Sweden.ORCID 0000-0002-4915-388X

Funding

Wellcome Trust
6 · The paper itself

Abstract

Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high throughput manner. The initial version requires the single photon counting modules for data acquisition, which limits its adoptability. Here, we present imaging-based SPP (iSPP) that can be performed by imaging a spot over time in the common imaging mode with confocal detectors. We also provide user-friendly software with a graphical user interface to analyze such data and give quantitative insights on the content and properties of nanoscale bioparticles. We use iSPP to decipher lipid-protein interactions, membrane modifications by drugs, and the heterogeneity of extracellular vesicles isolated from cell lines and human urine. This easily applicable modality of the single particle profiler will facilitate nanoscale bioparticle research in laboratories with access to any confocal microscope.

Indexed as

Extracellular VesiclesLipoproteinsLiposomesNanoparticlesHumansMicroscopy, ConfocalSoftwareLipoproteinsLiposomesconfocal microscopyenvironmental sensitive probesexosomesextracellular vesiclesliposomesmembrane fluiditysingle particle profiling

Identifiers

PMID39878336
PMCPMC11827106

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.