ReviewEngineering in life sciences2025
Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations.
Review in Engineering in life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Biosynthesis of cyanophycin by high-cell-density cultivation of recombinant Escherichia coli for corrosion-resistant biomaterial applications.Bioprocess and biosystems engineering · 2026Article
- Towards a Scalable Production ofInternational journal of molecular sciences · 2026Article
- Bioreactor Technology for Medicinal Plant In Vitro Cultures: Systems, Applications, and Future Perspectives.Biology · 2026Review
- Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor-Like Performance.Biotechnology and bioengineering · 2026Article
- Emerging Sustainable Bioprocess for the Valorization of Agave Bagasse for Single-Cell Protein Production.Foods (Basel, Switzerland) · 2026Article
- Catalytically active inclusion bodies of Bacillus clausii laccase protein recombinantly produced in E. coli for dye decolorization.Microbial cell factories · 2026Article
- Online Monitoring of Oxygen Transfer Rate as a Versatile Tool for Fermentation Characterization and Optimization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Enhanced anti-EpCAM ScFv production and reduced acetate overflow in a BW25113-derived E. coli strain with ArcA and Pka deletions.Microbial cell factories · 2025Article
- Adjusting Aspergillus niger pellet diameter, population heterogeneity, and core architecture during shake flask cultivation.Biotechnology for biofuels and bioproducts · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile application for a wide range of cell types-from bacteria to mammalian cells. In recent decades, extensive research has been conducted on the shake flask, to not perform processes blindly but to gain a deeper understanding of the various process parameters, phenomena, and their impact on the process. Although the characterization of the shake flask is now well-established in literature, many publications show that this knowledge is often inadequately applied. Therefore, this review provides an overview of the current state of knowledge on various topics related to the shake flask. We first present the key process parameters and their influence on different physical phenomena, such as power input, the largely unknown in-phase/out-of-phase phenomenon, as well as temperature and mass transfer. Then, the most common online monitoring systems that have been established for shake flasks are discussed. Finally, various pitfalls that often arise from inadequate knowledge of handling shake flask cultivations are discussed and guidance on how to avoid them is provided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.