ArticleJournal of biological engineering2025
A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy.
Article in Journal of biological engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Toxic and useful: harnessing yeast killer toxins for product recovery.Microbial cell factories · 2026Article
- Early bioprocess development using Komagataella phaffii: application scenarios for the microTOM mini bioreactor system.Journal of biological engineering · 2026Article
- tRNA-Mediated Plasmid Stabilization for Antibiotic-Free Applications inACS synthetic biology · 2026Article
- Online Monitoring of Oxygen Transfer Rate as a Versatile Tool for Fermentation Characterization and Optimization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bioprocess Engineering for Cultivated Meat.Advances in biochemical engineering/biotechnology · 2026Review
- Effect of High-Speed Shaking on Oxygen Transfer in Shake Flasks.Biotechnology journal · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundShake flasks are essential tools in biotechnological development due to their cost efficiency and ease of use. However, a significant challenge is the miniaturization of process analytical tools to maximize information output from each cultivation. This study aimed to develop a respiration activity online measurement system via off-gas analysis, named "Transfer rate Online Measurement" (TOM), for determining the oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and the respiration quotient (RQ) in surface-aerated bioreactors, primarily targeting shake flasks.
resultsSensors for off-gas analysis were placed in a bypass system that avoids the shaking of the electronics and sensors. An electrochemical oxygen sensor and an infrared CO
conclusionsThe developed TOM system showcases a novel approach to measuring OTR, CTR, and RQ in shaken bioreactors. It offers a robust and accurate solution for respiration activity analysis. Due to its flexible design and tunable accuracy, it enables measurement in various applications and different shake flasks.
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Registered trials
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