Evidence map›Paper›PMID 39869972›Full record

ArticleVirology2025

A high-throughput, microplate reader-based method to monitor in vitro HIV latency reversal in the absence of flow cytometry.

Chantal Emade Nkwelle, Unique Stephens, Kimberly Liang, Joel Cassel, Joseph M Salvino, Luis J Montaner, Roland N Ndip, Seraphine N Esemu, Fidele Ntie-Kang, Ian Tietjen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Chantal Emade NkwelleDepartment of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon; Center for Drug Discovery, University of Buea, Buea, Cameroon.
Unique StephensThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA.
Kimberly LiangThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA.
Joel CasselThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA.
Joseph M SalvinoThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA.
Luis J MontanerThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA.
Roland N NdipDepartment of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.
Seraphine N EsemuDepartment of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea, Cameroon.
Fidele Ntie-KangCenter for Drug Discovery, University of Buea, Buea, Cameroon; Department of Chemistry, Faculty of Science, University of Buea, Buea, Cameroon; Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany. Electronic address: fidele.ntie-kang@ubuea.cm.
Ian TietjenThe Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA. Electronic address: itietjen@wistar.org.

Funding

Virus & Reservoirs CoreP30AI045008 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI DAVID S METZGER · 1999 to 2026
$78.6M
BEAT-HIV: Delaney Collaboratory to Cure HIV-1 Infection by Combination ImmunotherapyUM1AI164570 · NIAID · WISTAR INSTITUTE · PI Luis J Montaner, James L. Riley · 2021 to 2026
$34.7M
Gates Foundation INV-036848NIAID NIH HHS P30 AI045008NIAID NIH HHS UM1 AI164570
6 · The paper itself

Abstract

J-Lat cells are derivatives of the Jurkat CD4

Indexed as

Anti-HIV AgentsHigh-Throughput Screening AssaysHIV-1HIV InfectionsVirus LatencyCD4-Positive T-LymphocytesDepsipeptidesFlow CytometryGreen Fluorescent ProteinsHumansJurkat CellsPhorbol EstersVirus ActivationAnti-HIV AgentsDepsipeptidesGreen Fluorescent ProteinsPhorbol EstersprostratinromidepsinHIV latencyJ-Lat cell lineLatency-reversing agentsMicroplate readerResource-limited laboratories

Identifiers

PMID39869972
PMCPMC12911319

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.