ArticlePLoS neglected tropical diseases2025
The Japanese encephalitis virus NS1' protein facilitates virus infection in mosquitoes.
Article in PLoS neglected tropical diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Comparative vector competence analysis reveals differential Tembusu virus transmission efficiency between Aedes albopictus and Culex quinquefasciatus.Parasites & vectors · 2026Article
- Thioester-Containing Protein TEP27 inInternational journal of molecular sciences · 2025Article
- VEGFR-3 deficiency in astrocytes exacerbates Japanese encephalitis virus-induced neuroinflammation in mouse brains.Zoological research · 2025Article
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Authors and funding
19 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is known for its capacity to cause severe neurological disease in Asia. Neurotropic flaviviruses within the Japanese encephalitis (JE) serogroup possess the distinctive feature of expressing a unique nonstructural protein, NS1'. The NS1' protein consists of the full NS1 protein with an additional 52 amino acid extension at the C-terminus and has been demonstrated to exhibit virulence in mammalian hosts upon infection. However, the precise role of the NS1' protein in the mosquito vectors has yet to be elucidated. METHODOLOGY/PRINCIPAL
findingsIn this study, an NS1'-defective virus (rG66A) was engineered, and its effect on the infection of mosquito cells was investigated. The results demonstrated a significant reduction in the infectivity of the rG66A virus in mosquito cells by RT-qPCR, indicating that the absence of the NS1' protein impedes JEV replication in Culex mosquitoes. Additionally, this research elucidated the underlying mechanism by which the NS1' protein enhances viral infection in mosquitoes by RNA-Seq analysis. Specifically, the NS1' protein was found to facilitate infection through the suppression of antimicrobial peptides (AMPs) regulated by the Toll pathway. CONCLUSIONS/SIGNIFICANCE: Our research demonstrated that the JEV NS1' protein contributes to immune escape, thereby enhancing viral infection in mosquitoes. This finding offers new insights into the transmission mechanisms of JEV, elucidating novel aspects of viral propagation.
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