Evidence map›Paper›PMID 39869566›Full record

ArticlePloS one2025

Impact of Chlorella vulgaris and probiotic supplementation on performance, immunity and intestinal microbiota of broiler chickens.

Verena Pereira Dinalli, Marcio Carvalho Costa, Emerson José Venâncio, João Antônio Barbosa Filho, José Antônio Bessegatto, Augusto Tasch Holkem, Amauri Alcindo Alfieri, Caio Abercio da Silva, Alexandre Oba

Abstract read
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Verena Pereira DinalliDepartment of Animal Science, State University of Londrina (UEL), Londrina, Paraná, Brazil.
Marcio Carvalho CostaDepartment of Biomedical Sciences, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
Emerson José VenâncioDepartment of Pathological Sciences, State University of Londrina (UEL), Londrina, Paraná, Brazil.ORCID 0000-0002-3735-9207
João Antônio Barbosa FilhoDepartment of Animal Science, State University of Londrina (UEL), Londrina, Paraná, Brazil.ORCID 0000-0002-0236-4380
José Antônio BessegattoLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, State University of Londrina (UEL), Londrina, Paraná, Brazil.
Augusto Tasch HolkemDepartment of Biomedical Sciences, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
Amauri Alcindo AlfieriLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, State University of Londrina (UEL), Londrina, Paraná, Brazil.
Caio Abercio da SilvaDepartment of Animal Science, State University of Londrina (UEL), Londrina, Paraná, Brazil.
Alexandre ObaDepartment of Animal Science, State University of Londrina (UEL), Londrina, Paraná, Brazil.ORCID 0000-0002-7706-9423

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chlorella vulgaris has antioxidant, antimicrobial, and anti-inflammatory properties, as well as the probiotic that is important for keeping the intestinal microbiota balanced. The objective was to test the impact of supplementation with microalgae and/or probiotics on broiler chickens' performance, immunity, and intestinal microbiota. The experimental design was in randomized blocks in a 4x2 factorial scheme, with four levels of inclusion of C. vulgaris (0; 0.25; 0.50 and 1%) associated or not with a commercial probiotic with five replications of 26 chickens per experimental unit. The results showed that probiotics improved feed conversion. Probiotics increased the productivity index only at 0.25% C. vulgaris supplementation. There was a reduction in spleen weight at 42 days of age in chickens fed with probiotics, but the different treatments did not alter serum antibodies. Sampling age had a significant impact on richness addressed by the number of observed genera and diversity addressed by the Shannon index. The most abundant phylum in the chicken intestinal tract was Firmicutes followed by Bacteroidetes and Proteobacteria. Bifidobacterium spp. was found in animals receiving 1% microalgae and probiotics on day 42, suggesting that this genus has benefited from microalgae supplementation. It is concluded that the probiotic and C. vulgaris have the potential to improve performance without causing major changes in the immunity and cecal microbiota.

Indexed as

ChickensChlorella vulgarisDietary SupplementsGastrointestinal MicrobiomeProbioticsAnimal FeedAnimalsSpleen

Identifiers

PMID39869566
PMCPMC11771937

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.