ArticleMicrobiology spectrum2025
Rapid, sensitive, and visible RPA-LFD assay for BoHV-1 and BoHV-5.
Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Research progress on the diagnostic techniques and vaccine development of bovine herpesvirus types 1.Frontiers in veterinary science · 2025Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Bovine herpesvirus (BoHV) infection poses a significant threat to the healthy development of the cattle industry. BoHV-1 primarily causes infectious bovine rhinotracheitis, while BoHV-5 is associated with bovine necrotic meningoencephalitis. These two pathogens not only exhibit a high correlation in antigenicity and genetic background but, more importantly, can establish latent infections within the bovine ganglion. Therefore, establishing a rapid and convenient pathogen detection method for BoHV-1 and BoHV-5 is crucial for early diagnosis, epidemic monitoring, and minimizing economic losses. In this study, utilizing the conserved sequences of BoHV-1 and BoHV-5 as targets, we developed a rapid and visually accessible recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay suitable for on-site detection. This method enables visual detection within 30 min for clinical samples containing as little as 1 tissue culture infectious dose 50 (TCID IMPORTANCE: Bovine herpesvirus (BoHV), a significant respiratory pathogen in cattle, is widely prevalent worldwide, posing a substantial economic threat to the cattle industry. In this study, we have developed a rapid on-site detection method for BoHV, termed RPA-LFD, which can carry out pathogen detection with a sensitivity of no less than 1 TCID
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