Evidence map›Paper›PMID 39868341›Full record

ArticlebioRxiv : the preprint server for biology2025

Extensive length and homology dependent chimerism in pool-packaged AAV libraries.

Jean-Benoît Lalanne, John K Mich, Chau Huynh, Avery C Hunker, Troy A McDiarmid, Boaz P Levi, Jonathan T Ting, Jay Shendure

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jean-Benoît LalanneDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.ORCID 0000-0001-8753-0669
John K MichAllen Institute for Brain Science, Seattle, WA, USA.
Chau HuynhDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.
Avery C HunkerAllen Institute for Brain Science, Seattle, WA, USA.
Troy A McDiarmidDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.
Boaz P LeviAllen Institute for Brain Science, Seattle, WA, USA.
Jonathan T TingAllen Institute for Brain Science, Seattle, WA, USA.
Jay ShendureDepartment of Genome Sciences, University of Washington, Seattle, WA, USA.

Funding

Open-Access AAV Toolbox for Basal Ganglia Cell Types and CircuitsUF1MH128339 · NIMH · ALLEN INSTITUTE · PI BAKKEN, TRYGVE, DAIGLE, TANYA LYNN · 2021 to 2021
$7.3M
Versatile, exponentially scalable methods for single cell molecular profilingR01HG010632 · NHGRI · UNIVERSITY OF WASHINGTON · PI Jay Ashok Shendure, Bruce Colston Trapnell · 2019 to 2026
$5.9M
NHGRI NIH HHS R01 HG010632NIMH NIH HHS UF1 MH128339
6 · The paper itself

Abstract

Adeno-associated viruses (AAVs) have emerged as the foremost gene therapy delivery vehicles due to their versatility, durability, and safety profile. Here we demonstrate extensive chimerism, manifesting as pervasive barcode swapping, among complex AAV libraries that are packaged as a pool. The observed chimerism is length- and homology-dependent but capsid-independent, in some cases affecting the majority of packaged AAV genomes. These results have implications for the design and deployment of functional AAV libraries in both research and clinical settings.

Identifiers

PMID39868341
PMCPMC11761685

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.