Evidence map›Paper›PMID 39868182›Full record

ArticlebioRxiv : the preprint server for biology2025

Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection.

Silvia Benito-Martínez, Laura Salavessa, Anne-Sophie Macé, Nathan Lardier, Vincent Fraisier, Julia Sirés-Campos, Riddhi Atul Jani, Maryse Romao, Charlène Gayrard, Marion Plessis and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Silvia Benito-MartínezInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Laura SalavessaInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.ORCID 0000-0002-7386-8535
Anne-Sophie MacéInstitut Curie, PSL Research University, CNRS, UMR144, Cell and Tissue Imaging Facility (PICT-IBiSA), 75005 Paris, France.
Nathan LardierInstitut Curie, PSL Research University, CNRS, UMR144, Molecular Mechanisms of Intracellular Transport, 75005 Paris, France.
Vincent FraisierInstitut Curie, PSL Research University, CNRS, UMR144, Cell and Tissue Imaging Facility (PICT-IBiSA), 75005 Paris, France.
Julia Sirés-CamposInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Riddhi Atul JaniInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Maryse RomaoInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Charlène GayrardL'Oréal Research and Innovation, Aulnay-sous-Bois, France.
Marion PlessisL'Oréal Research and Innovation, Aulnay-sous-Bois, France.
Ilse HurbainInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Cécile Nait-MeddourUniv Paris Est Creteil, INSERM, IMRB, Translational Neuropsychiatry, F-94010 Creteil, France.
Etienne MorelUniversité Paris Cité, INSERM UMR-S1151, CNRS UMR-S8253, Institut Necker Enfants Malades, F-75015 Paris, France.ORCID 0000-0002-4763-4954
Michele BoniottoUniv Paris Est Creteil, INSERM, IMRB, Translational Neuropsychiatry, F-94010 Creteil, France.
Jean-Baptiste MannevilleInstitut Curie, PSL Research University, CNRS, UMR144, Molecular Mechanisms of Intracellular Transport, 75005 Paris, France.
Françoise BernerdL'Oréal Research and Innovation, Aulnay-sous-Bois, France.
Christine DuvalL'Oréal Research and Innovation, Aulnay-sous-Bois, France.
Graça RaposoInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Cédric DelevoyeInstitut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.ORCID 0000-0002-3835-6649

Funding

Hermansky Pudlak Syndrome &melanosome formationR01EY015625 · NEI · UNIVERSITY OF PENNSYLVANIA · PI Michael S Marks · 2004 to 2026
$9.2M
NEI NIH HHS R01 EY015625
6 · The paper itself

Abstract

Melanin pigments block genotoxic agents by positioning on the sun-exposed side of human skin keratinocytes' nucleus. How this position is regulated and its role in genome photoprotection remains unknown. By developing a model of human keratinocytes internalizing extracellular melanin into pigment organelles, we show that keratin 5/14 intermediate filaments mechanically control the 3D perinuclear position of pigments, shielding DNA from photodamage. Imaging and microrheology in human disease-related model identify structural keratin cages surrounding pigment organelles to stiffen their microenvironment and maintain their 3D position. Optimum pigment spatialization is required for DNA photoprotection and rely on the interplay between intermediate filaments and microtubules bridged by plectin cytolinkers. Thus, the mechanically-driven proximity of pigment organelles to the nucleus is a key photoprotective parameter. Uncovering how human skin counteracts solar radiation by positioning the melanin microparasol next to the genome anticipates that dynamic spatialization of organelles is a physiological UV stress response.

Indexed as

intermediate filamentskeratinocytekeratinsmelaninmicrotubulesorganelle distributionskin pigmentation and photoprotection

Identifiers

PMID39868182
PMCPMC11761041

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.