ArticleOptica2021
Instant FLIM enables 4D
Article in Optica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Large-FOV, dual-region, two-photon fluorescence lifetime imaging microscopy (LD-2P-FLIM) for multiparameter and quantitative brain function imaging.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.bioRxiv : the preprint server for biology · 2026Article
- Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Absolute measurement of fast and slow neuronal signals with fluorescence lifetime photometry at high temporal resolution.bioRxiv : the preprint server for biology · 2025Article
- Chronic compression induces transcriptional, metabolic, and functional state changes in macrophages that recapitulate tumor-associated phenotypes.Frontiers in immunology · 2025Article
- Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.Communications biology · 2024Article
- Light-field tomographic fluorescence lifetime imaging microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Imaging immunometabolismImmunometabolism (Cobham, Surrey) · 2024Article
- Fast and slow: Recording neuromodulator dynamics across both transient and chronic time scales.Science advances · 2024Article
- Light-field tomographic fluorescence lifetime imaging microscopy.Research square · 2023Article
- Direct frequency domain fluorescence lifetime imaging using simultaneous ultraviolet and visible excitation.Biomedical optics express · 2023Article
- Fluorescence lifetime tracking and imaging of single moving particles assisted by a low-photon-count analysis algorithm.Biomedical optics express · 2023Article
- Small training dataset convolutional neural networks for application-specific super-resolution microscopy.Journal of biomedical optics · 2023Article
- Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks.Frontiers in bioinformatics · 2023Article
- Real-time open-source FLIM analysis.Frontiers in bioinformatics · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Traditional fluorescence microscopy is blind to molecular microenvironment information that is present in fluorescence lifetime, which can be measured by fluorescence lifetime imaging microscopy (FLIM). However, most existing FLIM techniques are slow to acquire and process lifetime images, difficult to implement, and expensive. Here, we present instant FLIM, an analog signal processing method that allows real-time streaming of fluorescence intensity, lifetime, and phasor imaging data through simultaneous image acquisition and instantaneous data processing. Instant FLIM can be easily implemented by upgrading an existing two-photon microscope using cost-effective components and our open-source software. We further improve the functionality, penetration depth, and resolution of instant FLIM using phasor segmentation, adaptive optics, and super-resolution techniques. We demonstrate through-skull intravital 3D FLIM of mouse brains to depths of 300 μm and present the first
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.