Evidence map›Paper›PMID 39866894›Full record

ArticleWorld journal of stem cells2025

Immortalization of epidural fat-derived mesenchymal stem cells:

Seoung-Woo Lee, Young-Ju Lim, Hee-Yeon Kim, Wansoo Kim, Wook-Tae Park, Min-Jung Ma, Junho Lee, Min-Soo Seo, Young In Kim, Sangbum Park and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of stem cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Seoung-Woo LeeCore Protein Resources Center, Daegu-Gyeongbuk Institute of Science and Technology, Daegu 42988, South Korea.
Young-Ju LimDepartment of Orthopedic Surgery, Yeungnam University Medical Center, Yeungnam University College of Medicine, Daegu 42415, South Korea.
Hee-Yeon KimDepartment of Veterinary Pathology, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, South Korea.
Wansoo KimSchool of Life Science, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, South Korea.
Wook-Tae ParkDepartment of Orthopedic Surgery, Yeungnam University Medical Center, Yeungnam University College of Medicine, Daegu 42415, South Korea.
Min-Jung MaLaboratory of Veterinary Tissue Engineering, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, South Korea.
Junho LeeCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, South Korea.
Min-Soo SeoDepartment of Veterinary Tissue Engineering, Kyungpook National University, Daegu 41566, South Korea.
Young In KimCellexobio., Ltd., Daegu 42415, South Korea.
Sangbum ParkMichigan State University, Institute for Quantitative Health Science & Engineering, East Lansing, MI 48824, United States.
Seong-Kyoon ChoiCore Protein Resources Center, Daegu-Gyeongbuk Institute of Science and Technology, Daegu 42988, South Korea.
Gun Woo LeeDepartment of Orthopedic Surgery, Yeungnam University College of Medicine, Daegu 42415, South Korea. gwlee1871@gmail.com.

Funding

Understanding immune-epithelial interactions during wound repair in live mammalsR01AR083086 · NIAMS · MICHIGAN STATE UNIVERSITY · PI Sangbum Park · 2023 to 2026
$1.9M
NIAMS NIH HHS R01 AR083086
6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) are promising candidates for regenerative therapy due to their self-renewal capability, multilineage differentiation potential, and immunomodulatory effects. The molecular characteristics of MSCs are influenced by their location. Recently, epidural fat (EF) and EF-derived MSCs (EF-MSCs) have garnered attention due to their potential benefits to the spinal microenvironment and their high expression of neural SC markers. However, their clinical applications are limited due to cell senescence and limited accessibility of EF. Although many studies have attempted to establish an immortalized, stable SC line, the characteristics of immortalized EF-MSCs remain to be clarified.

aimTo establish and analyze stable immortalized EF-MSCs.

methodsThe phenotypes of EF-MSCs were analyzed using optical microscopy. Cell immortalization was performed using lentiviral vectors. The biomolecular characteristics of the cells were analyzed by immunoblotting, quantitative PCR, and proteomics.

resultsThe immortalized EF-MSCs demonstrated a significantly extended lifespan compared to the control group, with well-preserved adipogenic potential and SC surface marker expression. Introduction of human telomerase reverse transcriptase genes markedly increased the lifespan of EF-MSCs. Proteomics analysis revealed substantial increase in the expression of DNA replication pathway components in immortalized EF-MSCs.

conclusionImmortalized EF-MSCs exhibited significantly enhanced proliferative capacity, retained adipogenic potential, and upregulated the expression of DNA replication pathway components.

Indexed as

Epidural fatImmortalizationProteomicsStem cellTransfection

Identifiers

PMID39866894
PMCPMC11752455

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.