Evidence map›Paper›PMID 39865375›Full record

ArticleProtein science : a publication of the Protein Society2025

A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hH

Emerson M Carmona, D Marien Cortes, Luis G Cuello

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Emerson M CarmonaCell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.ORCID 0000-0003-1332-0372
D Marien CortesCell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.ORCID 0000-0001-9733-0679
Luis G CuelloCell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.ORCID 0000-0001-5192-7568

Funding

A comprehensive thermodynamic and structural characterization of ion channel function and its regulation by the lipid bilayer composition.R35GM149523 · NIGMS · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI Luis Gonzalo Cuello · 2023 to 2026
$1.8M
Comisión Nacional de Investigación Científica y Tecnológica (CONICYT)-Programa Formación de Capital Humano Avanzado (PFCHA) 2017-21170395NIGMS NIH HHS 1R35GM149523-02NIGMS NIH HHS R35 GM149523Pew Charitable TrustsWelch Foundation BI-194
6 · The paper itself

Abstract

Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH

Indexed as

Ion ChannelsEscherichia coliGene ExpressionHumansProtein StabilityRecombinant ProteinsSolubilityHVCN1 protein, humanIon ChannelsRecombinant Proteinsdetergent screeningmembrane protein purificationoverexpressionvoltage‐gated proton channel (Hv1)

Identifiers

PMID39865375
PMCPMC11761714

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.