Evidence map›Paper›PMID 39864310›Full record

ArticleBiosensors & bioelectronics2025

Mitigating dithiothreitol interference to gold/thiol interface in electrochemical detection of cathepsin B activity toward multiplex protease analysis.

Bingun Habarakadage, Sabari Rajendran, Zhaoyang Ren, Morgan J Anderson, Jessica Koehne, Lingaraju Gorla, Shunya Morita, Sara Wu, Duy H Hua, Jun Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Bingun HabarakadageDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Sabari RajendranDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Zhaoyang RenDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Morgan J AndersonMillennium Integration and Engineering Services (MEIS), Moffett Field, CA, 94035, USA; NASA Ames Research Center, Moffett Field, CA, 94035, USA.
Jessica KoehneNASA Ames Research Center, Moffett Field, CA, 94035, USA.
Lingaraju GorlaDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Shunya MoritaDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Sara WuNew Hope BioDiagnostics, LLC, 193 Amber Wood Run, Chapel Hill, NC, 27516, USA.
Duy H HuaDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA.
Jun LiDepartment of Chemistry, Kansas State University, Manhattan, KS, 66502, USA. Electronic address: junli@ksu.edu.

Funding

Rapid Protease Profiling with a Multiplex Electronic Method for Detection of Metastatic Triple-Negative Breast CancerR01CA217657 · NCI · KANSAS STATE UNIVERSITY · PI LI, JUN · 2017 to 2020
$1.4M
NCI NIH HHS R01 CA217657
6 · The paper itself

Abstract

Proteases are overexpressed at various stages of conditions such as cancers and thus can serve as biomarkers for disease diagnosis. Electrochemical techniques to detect the activity of extracellular proteases have gained attraction due to their multiplexing capability. Here we employ an electrochemical approach based on a 3 × 3 gold (Au) microelectrode array (MEA) functionalized with (2-aminoethyl)ferrocene (AEF) tagged specific peptide substrates to monitor cathepsin B (CB) protease activity. Cleavage of these peptide substrates by proteases leads to an exponential decay in the alternating current voltammetry (ACV) signal. The protease activity is represented by the inverse of the decay time constant (1/τ), which is equal to (k

Indexed as

Biosensing TechniquesCathepsin BDithiothreitolElectrochemical TechniquesGoldFerrous CompoundsHumansMetallocenesMicroelectrodesPeptidesSulfhydryl CompoundsCathepsin BDithiothreitolferroceneFerrous CompoundsGoldMetallocenesPeptidesSulfhydryl CompoundsCathepsin BDithiothreitolElectrochemical stabilityMultiplex detectionProteolysis activitySelf-assembled monolayer

Identifiers

PMID39864310
PMCPMC11795661

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.