Evidence map›Paper›PMID 39863814›Full record

Observational studyEuropean archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery2025

Circulating HPVDNA in patients undergoing transoral robotic surgery for oropharyngeal cancer: liquid biopsy could identify molecular residual disease.

Flaminia Campo, Francesca Paolini, Irene Terrenato, Giovanni Blandino, Valentina De Pascale, Oreste Iocca, Silvia Moretto, Valentina Manciocco, Antonello Vidiri, Aldo Venuti and 1 more

Erratum issuedAbstract readObservational Study
PubMed Publisher
In one paragraph

Observational study in European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Explore the role of ctHPVDNA kinetics during diagnosis and follow-up of HPV OPSCC.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Flaminia CampoDepartment of Otolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Via Elio Chianesi 53, 00144, Rome, Italy. flaminiacampo@gmail.com.ORCID http://orcid.org/0000-0002-9249-7573
Francesca PaoliniHPV - Unit, UOSD Tumor Immunology and Immunotherapy IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Rome, Italy.
Irene TerrenatoClinical Trial Center - Biostatistics & Bioinformatics, IRCCS Regina Elena National Cancer Institute, Rome, Italy.
Giovanni BlandinoOncogemonic and Epigenetic Unit, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Rome, Italy.
Valentina De PascaleTranslational Oncologic Research, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Rome, Italy.
Oreste IoccaDivision of Maxillofacial Surgery, Department of Surgical Science, University of Torino, Torino, Italy.
Silvia MorettoDepartment of Otolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Via Elio Chianesi 53, 00144, Rome, Italy.
Valentina MancioccoDepartment of Otolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Via Elio Chianesi 53, 00144, Rome, Italy.
Antonello VidiriDepartment of Radiology and Diagnostic Imaging, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Rome, Italy.
Aldo VenutiHPV - Unit, UOSD Tumor Immunology and Immunotherapy IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Rome, Italy.
Raul PelliniDepartment of Otolaryngology-Head and Neck Surgery, IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO), Via Elio Chianesi 53, 00144, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveswe evaluated the hypothesis that level of ctHPVDNA on the first postoperative day (POD-1); and at 15 days (POD-15) could be associated with the need for adjuvant therapy and the presence of recurrence. MATERIALS AND

methodsthis is a prospective observational study on biomarkers, focusing on the longitudinal monitoring of ctHPVDNA in a cohort of HPV-OPSCC patients undergoing TORS. Blood samples were collected according to the following schema: (1) pretreatment; (2) on first postoperative day (POD 1); and (3) at 15 days (POD 15). Plasma samples were analyzed with ddPCR assay comprising E6 of HPV16, HPV 33 and HPV 35.

resultsPresent study was conducted on 44 OPSCC patients and revealed a ctHPVDNA sensitivity of 100% (95%CI: 89-100%) in blood samples at first diagnosis. Data demonstrated a significant different of ctHPVDNA levels at POD-1 among patients who received observation vs. adjuvant treatment and among patients who remained disease-free at the last follow-up, compared to those who experienced recurrence. In the next years, studies on larger patients' surgical cohorts focused on ctHPVDNA levels at POD-1 and continued improvements in assay methodology could allow the implementation of ctHPVDNA in routine clinical use.

conclusionLiquid biopsy could identify residual molecular disease after surgery and guide clinicians choosing adjuvant treatment.

Indexed as

DNA, ViralOropharyngeal NeoplasmsPapillomavirus InfectionsRobotic Surgical ProceduresAdultAgedBiomarkers, TumorFemaleHumansLiquid BiopsyMaleMiddle AgedNeoplasm Recurrence, LocalNeoplasm, ResidualProspective StudiesBiomarkers, TumorDNA, ViralCirculating HPV DNADe-escalation therapyLiquid biopsyOropharyngeal cancerTORS

Identifiers

PMID39863814

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.