Label-Free Surface-Enhanced Raman Scattering for Genomic DNA Cytosine Methylation Reading.

Kazi Morshed Alom, Anastasiia Tukova, Nana Lyu, Alison Rodger, Yuling Wang

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Occurrence, Properties, Applications and Analytics of Cytosine and Its Derivatives.Molecules (Basel, Switzerland) · 2025
Review
SERS-based detection of DNA methylation for cancer diagnosis: Cation-mediated adsorption to silver nanoparticles.PloS one · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kazi Morshed AlomSchool of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0001-7332-6405

Anastasiia TukovaSchool of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0003-0316-1814

Nana LyuSchool of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia.

Alison RodgerResearch School of Chemistry, The Australian National University, Canberra, ACT 2601, Australia.ORCID 0000-0002-7111-3024

Yuling WangSchool of Natural Sciences, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0003-3627-7397

Funding

Australian Research Council DP220102086

6 · The paper itself

Abstract

DNA methylation has been widely studied with the goal of correlating the genome profiles of various diseases with epigenetic mechanisms. Multiple approaches have been developed that employ extensive steps, such as bisulfite treatments, polymerase chain reactions (PCR), restriction digestion, sequencing, mass analysis, etc., to identify DNA methylation. In this article, we report a facile label-free surface-enhanced Raman scattering (SERS) spectroscopy system that utilizes gold nanoparticles (AuNPs) for signal enhancement of methylated DNA. The key innovation of this work is to use anionic nanoparticles at a high ionic strength to introduce the aggregation of AuNPs with anionic DNA. When target methylated DNA is present, the presence of a methyl group in the cytosine C5 position of CpG sites induces a Raman peak at 1350 cm

Indexed as

CytosineDNADNA MethylationSpectrum Analysis, RamanCell Line, TumorCpG IslandsGoldHumansLimit of DetectionMetal NanoparticlesCytosineDNAGoldCpG siteDNA methylationgold nanoparticleslabel-free surface-enhanced Raman scattering (SERS)

Identifiers

PMID39860272

DOI10.3390/molecules30020403

PMCPMC11767753

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.