Pyrrolysine Aminoacyl-tRNA Synthetase as a Tool for Expanding the Genetic Code.

Anastasia Dakhnevich, Alisa Kazakova, Danila Iliushin, Roman A Ivanov

Abstract readReview

In one paragraph

Review in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives.Frontiers in molecular biosciences · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anastasia DakhnevichBiotechnology Department, Sirius University of Science and Technology, 354349 Sirius, Russia.

Alisa KazakovaBiotechnology Department, Sirius University of Science and Technology, 354349 Sirius, Russia.

Danila IliushinBiotechnology Department, Sirius University of Science and Technology, 354349 Sirius, Russia.ORCID 0009-0002-7092-8376

Roman A IvanovBiotechnology Department, Sirius University of Science and Technology, 354349 Sirius, Russia.ORCID 0000-0002-9573-4183

Funding

Ministry of Science and Higher Education of the Russian Federation 075-10-2021-093

6 · The paper itself

Abstract

In addition to the 20 canonical amino acids encoded in the genetic code, there are two non-canonical ones: selenocysteine and pyrrolysine. The discovery of pyrrolysine synthetases (PylRSs) was a key event in the field of genetic code expansion research. The importance of this discovery is mainly due to the fact that the translation systems involving PylRS, pyrrolysine tRNA (tRNA

Indexed as

Amino Acyl-tRNA SynthetasesGenetic CodeLysineHumansProtein BiosynthesisAmino Acyl-tRNA SynthetasesLysinepyrrolysinegenetic code expansionorthogonal pairspyrrolysine aminoacyl-tRNA synthetasetRNA engineering

Identifiers

PMID39859254

DOI10.3390/ijms26020539

PMCPMC11764691

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.