Luminescence Properties of Hoechst 33258 in Polyvinyl Alcohol Films.

Bong Lee, Agnieszka Jablonska, Danh Pham, Rajveer Sagoo, Zygmunt Gryczynski, Trang Thien Pham, Ignacy Gryczynski

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Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Bong LeeDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.

Agnieszka JablonskaDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.ORCID 0009-0007-5461-7830

Danh PhamDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.ORCID 0000-0003-0274-9640

Rajveer SagooDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.ORCID 0009-0000-8676-1633

Zygmunt GryczynskiDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.

Trang Thien PhamDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.ORCID 0009-0004-8038-5082

Ignacy GryczynskiDepartment of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report a comprehensive investigation of the photophysical properties of Hoechst 33258 (HOE) embedded in polyvinyl alcohol (PVA) films. HOE displays a bright, highly polarized, blue fluorescence emission centered at 430 nm, indicating effective immobilization within the polymer matrix of PVA. Its fluorescence quantum yield is notably high (~0.74), as determined relative to a quinine sulfate standard. In addition, we observed that HOE-doped PVA films exhibit room temperature phosphorescence (RTP) that remains visible for several seconds after UV excitation ceases. The slightly negative phosphorescence anisotropy implies that the triplet-singlet radiative transition is orthogonal to the singlet-singlet transition governing fluorescence. Notably, we observed that direct triplet-state excitation at longer wavelengths (beyond the primary absorption band) produces highly polarized RTP. We believe this possibility of direct triplet-state excitation opens new avenues for studying RTP in polymer-immobilized molecules.

Indexed as

BisbenzimidazoleLuminescencePolyvinyl AlcoholLuminescent MeasurementsBisbenzimidazolePolyvinyl Alcoholdirect triplet excitationHoechstlong-lived phosphorescenceroom temperature phosphorescence

Identifiers

PMID39859229

DOI10.3390/ijms26020514

PMCPMC11764979

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