Evidence map›Paper›PMID 39857952›Full record

ArticleCancers2025

Expression Pattern of AIFM3, VGLL4, and WNT4 in Patients with Different Stages of Colorectal Cancer.

Danijel Bevanda, Anita Racetin, Nela Kelam, Natalija Filipović, Mateo Bevanda, Marina Rudan Dimlić, Jelena Budimir, Daniela Bevanda Glibo, Ivana Bevanda, Danica Ramljak and 1 more

Abstract read
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In one paragraph

Article in Cancers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Danijel BevandaDepartment of Gastroenterology, School of Medicine, University of Mostar, University Hospital Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina.
Anita RacetinDepartment of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split School of Medicine, Šoltanska 2A, 21000 Split, Croatia.
Nela KelamDepartment of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split School of Medicine, Šoltanska 2A, 21000 Split, Croatia.ORCID 0000-0002-6529-5474
Natalija FilipovićDepartment of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split School of Medicine, Šoltanska 2A, 21000 Split, Croatia.ORCID 0000-0002-8943-4109
Mateo BevandaDepartment of Surgery, School of Medicine, University of Mostar, University Hospital Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina.ORCID 0000-0002-2121-4302
Marina Rudan DimlićMediterranean Institute for Life Sciences (MedILS), University of Split, Meštrovićevo Šetalište 45, 21000 Split, Croatia.
Jelena BudimirMediterranean Institute for Life Sciences (MedILS), University of Split, Meštrovićevo Šetalište 45, 21000 Split, Croatia.ORCID 0000-0001-5411-5530
Daniela Bevanda GliboDepartment of Gastroenterology, School of Medicine, University of Mostar, University Hospital Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina.
Ivana BevandaDepartment of Endocrinology, School of Medicine, University of Mostar, University Hospital Mostar, Bijeli Brijeg bb, 88000 Mostar, Bosnia and Herzegovina.
Danica RamljakMediterranean Institute for Life Sciences (MedILS), University of Split, Meštrovićevo Šetalište 45, 21000 Split, Croatia.
Katarina VukojevićDepartment of Anatomy, Histology and Embryology, Laboratory for Early Human Development, University of Split School of Medicine, Šoltanska 2A, 21000 Split, Croatia.ORCID 0000-0003-2182-2890

Funding

Croatian Science Foundation IP-2022-10-8720
6 · The paper itself

Abstract

BACKGROUND/

objectivesColorectal cancer (CRC) remains a significant health burden, and its delayed diagnosis at advanced stages leads to poor survival outcome. Detection of known and novel prognostic markers is essential. In this study, the status of likely prognostic markers-the apoptotic inducing factor (AIFM3), vestigial-like family member 4 (VGLL4), and WNT4-was evaluated.

methodsAIFM3, VGLL4, and WNT4 expression in CRC tissues across different stages (Dukes A-D) were analyzed using histological immunofluorescence staining and RNA sequencing analyses.

resultsIn advanced CRC stages, progressive loss of normal crypt architecture, reduction of goblet cells, and necrotic debris were detected along with differential expression patterns of AIFM3, VGLL4, and WNT4. AIFM3 exhibited high reactivity in the lamina propria of healthy tissue and Dukes A, but this was diminished in advanced CRC stages. VGLL4 expression, initially confined to the lamina propria, increased significantly in the epithelium of Dukes B and C, with a cytoplasmic localization pattern. WNT4 expression was elevated in the CRC epithelium across all stages, contrasting with a significant reduction in lamina propria reactivity. RNA sequencing corroborated these findings, showing significant downregulation of AIFM3 and WNT4 and upregulation of VGLL4 in CRC tissues compared to controls. Expression of AIFM3 and WNT4 showed no correlation with survival outcome, while low VGLL4 expression was correlated with better survival outcome.

conclusionsThe results suggest distinct roles for AIFM3, VGLL4, and WNT4 in CRC progression, highlighting only VGLL4 as a potential prognostic marker. Further evaluation of VGLL4 and its specific role in CRC progression remains to be elucidated.

Indexed as

AIFM3colorectal cancerVGLL4WNT4

Identifiers

PMID39857952
PMCPMC11763972

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.