Evidence map›Paper›PMID 39857443›Full record

ArticleAntioxidants (Basel, Switzerland)2025

Protective Effect of Conditioned Medium of Immortalized Human Stem Cells from Exfoliated Deciduous Teeth Against Hair Graying Caused by X-Ray Irradiation via Its Antioxidative Activity.

Yasuhiro Katahira, Eri Horio, Natsuki Yamaguchi, Jukito Sonoda, Miu Yamagishi, Satomi Miyakawa, Fumihiro Murakami, Hideaki Hasegawa, Izuru Mizoguchi, Takayuki Yoshimoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antioxidants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Special Issue "State-of-the-Art Cancer Immunotherapies-2nd Edition".International journal of molecular sciences · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yasuhiro KatahiraDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Eri HorioDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Natsuki YamaguchiDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Jukito SonodaDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Miu YamagishiDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Satomi MiyakawaDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Fumihiro MurakamiDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Hideaki HasegawaDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Izuru MizoguchiDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Takayuki YoshimotoDepartment of Immunoregulation, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.ORCID 0000-0002-2847-0341

Funding

Cysay Corporation
6 · The paper itself

Abstract

Hair graying is one of the common visible signs of human aging, resulting from decreased or abolished melanogenesis due to the depletion of melanocyte stem cells through excess accumulation of oxidative stress. Cell-free therapy using a conditioned medium (CM) of mesenchymal stem cells has been highlighted in the field of regenerative medicine owing to its potent therapeutic effects with lower regulatory hurdles and safety risk. Recently, we demonstrated that a CM of an immortalized stem cell line from human exfoliated deciduous teeth (SHED) has protective effects against a mouse model of ulcer formation via antioxidative and angiogenic activities mediated by HGF and VEGF. However, to date, no effective treatments for hair graying have been developed, and the effect of SHED-CM on hair graying remains unknown. In this study, we have investigated the effect of SHED-CM on a hair graying mouse model caused by X-ray irradiation. Repetitive subcutaneous administrations of SHED-CM greatly suppressed the development of hair graying, when compared to control medium, resulting in reduced cutaneous expression of 8-hydroxy-2'-deoxyguanosine, the major product of DNA damage induced by reactive oxygen species. Consistent with these in vivo results, SHED-CM significantly inhibited the cell death caused by X-ray irradiation in melanoma cell line B16F10 cells. Immunodepletion of HGF or VEGF in the SHED-CM revealed that this inhibition was due to suppression of the generation of reactive oxygen species, which was mainly mediated by HGF and probably VEGF. These results suggest that SHED-CM has protective effects against hair graying via its antioxidative activity.

Indexed as

antioxidative activitycell-free therapyconditioned mediumhair grayingstem cells from human exfoliated deciduous teeth

Identifiers

PMID39857443
PMCPMC11760446

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.