Evidence map›Paper›PMID 39856772›Full record

ArticleTropical medicine and health2025

Assessment of flavivirus RNA stability and infectivity in various water environments.

Yuka Sano, Hawraa Al-Alawi, Misao Himeno, Ryuichi Majima, Kazumi Haga, Myo Thura Kyaw, Satoshi Taniguchi, Meng Ling Moi

Abstract read
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In one paragraph

Article in Tropical medicine and health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuka Sano *School of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Hawraa Al-Alawi *Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.
Misao HimenoSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Ryuichi MajimaSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Kazumi HagaSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Myo Thura KyawSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Satoshi TaniguchiSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan.
Meng Ling MoiSchool of International Health, Graduate School of Medicine, the University of Tokyo, Tokyo, 113-0033, Japan. sherry@m.u-tokyo.ac.jp.

Funding

Japan Agency for Medical Research and Development (AMED) P233fa627001, JP22fk0108123 and 23wm0225033h0001.
6 · The paper itself

Abstract

introductionFlaviviruses such as dengue virus (DENV), Zika virus (ZIKV), Japanese encephalitis virus (JEV), and Yellow fever virus (YFV) are mosquito-borne RNA viruses causing major public health threats in major parts of the world. While DENV and ZIKV have been detected in urine samples, data on the presence and stability of flaviviruses in the water environment are limited.

methodsIn this study, we determined the stability and infectivity of flavivirus in different water environments by utilizing RT-qPCR and plaque assay to explore the feasibility of environmental detection and surveillance of flaviviruses.

resultsViral RNA could be detected for up to 49-days, at 4 °C, 25 °C and 37 °C temperatures, and infectious particles could be detected for up to 7 days. While our findings showed that flaviviral RNA has higher stability and better detection rates at lower temperatures, the infectious capacity of flaviviruses was comparatively short at 7 days.

conclusionsOur results indicate that flaviviruses retains limited infectivity in general water environments and highlight the feasibility of detection and surveillance in various epidemiologic and environmental settings.

Indexed as

DengueEnvironmentJapanese encephalitisOrthoflavivirusRNA virusSurveillanceWaterYellow feverZika

Identifiers

PMID39856772
PMCPMC11763117

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.