ArticleCommunications biology2025
Methylene blue at recommended concentrations alters metabolism in early zebrafish development.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
5 citing papers in PubMed.
- Acute administration of Manning compound during the spawning period reduces reproductive success in female zebrafish.Frontiers in endocrinology · 2026Article
- Plating larval zebrafish prior to the day of experimentation has no impact on spontaneous swimming and startle responses.MethodsX · 2025Article
- Durian peel-seed biochar for efficient methylene blue removal from water: synthesis, characterization, and adsorption performance.RSC advances · 2025Article
- A comparison of normalization methods for the expression of genes associated with oxidative stress in the liver of sheep.BMC genomic data · 2025Article
- Exploring the impact of acute solvent exposure on larval zebrafish behaviour.Frontiers in behavioral neuroscience · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methylene blue (MB) is an antifungal agent widely used during critical stages of zebrafish development. Most guidelines recommend 0.00005% or 0.0001% of MB for embryo/larval rearing. The Organisation for Economic Co-operation and Development zebrafish embryo toxicity test guideline omits MB recommendations, leading to inconsistent MB use in zebrafish research. Because MB affects oxidative energy metabolism in vitro and in vivo, we investigate possible metabolic effects of recommended MB concentrations in developing zebrafish (1-5 days post-fertilization (dpf)). MB increases O
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Registered trials
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