Evidence map›Paper›PMID 39856167›Full record

ArticleScientific reports2025

Scalable preparation of furanosteroidal viridin, β-viridin and viridiol from Trichoderma virens.

Wen Zhang, Kazu Sunami, Shuo Liu, Desita Triana, Zetryana Puteri Tachrim, Rikuto Kikuchi, Tohru Taniguchi, Kenji Monde, Tsunayoshi Takehara, Da-Yang Zhou and 4 more

Abstract read
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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Wen ZhangDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Kazu SunamiDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Shuo LiuDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Desita TrianaDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Zetryana Puteri TachrimDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Rikuto KikuchiGraduate School of Life Science, Hokkaido University, Kita 21 Nishi 11, Sapporo, 001-0021, Japan.
Tohru TaniguchiGraduate School of Life Science, Hokkaido University, Kita 21 Nishi 11, Sapporo, 001-0021, Japan.
Kenji MondeGraduate School of Life Science, Hokkaido University, Kita 21 Nishi 11, Sapporo, 001-0021, Japan.
Tsunayoshi TakeharaThe Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki-shi, 567-0047, Osaka, Japan.
Da-Yang ZhouThe Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki-shi, 567-0047, Osaka, Japan.
Takeyuki SuzukiThe Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki-shi, 567-0047, Osaka, Japan.
Yasuyuki HashidokoDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan.
Makoto HashimotoDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan. hasimoto@agr.hokudai.ac.jp.
Yuta MuraiDivision of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-8589, Hokkaido, Japan. ymurai@agr.hokudai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viridin and viridiol, along with wortmannin, metabolized by filamentous fungus Trichoderma virens, are identical furanosteroids with high-potent inhibitory activity towards phosphatidylinositol 3-kinase (PI3K) that associates the growth of tumor cells. Therefore, structure-activity relationship study (SAR) of these furanosteroids contributes to the development of novel drugs. However, rational supply methods have not been established yet. In this study, we generated an efficient method to produce both viridin and viridiol by using a unique pH regulated T. virens culture manner. Besides, we successfully obtained β-viridin (epimer of viridin) crystal and X-ray structure, of which the CAS number was registered without stereochemistry. Furthermore, applying the original method to stable isotope study, we also obtained [U-

Indexed as

FuransSterolsTrichodermaCrystallography, X-RayStructure-Activity RelationshipWortmanninFuransSterolsWortmanninTrichoderma virens[U-13C]-viridin[U-13C]-viridolViridinViridiolΒ-viridin

Identifiers

PMID39856167
PMCPMC11760887

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.