Evidence map›Paper›PMID 39856131›Full record

ArticleScientific reports2025

Influence of different priming treatments on germination potential and seedling establishment of four important hemp (Cannabis sativa L.) cultivars.

Saba Latif, Rahmatullah Qureshi, Abdul Rauf, NoshinIlyas, Qaiser Hussain, Syed Sabir Hussain Shah, Sabith Rehman, Arshad Mahmood Khan, Nasrullah Khan, Mostafa A Abdel-Maksoud and 3 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Saba LatifDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan.
Rahmatullah QureshiDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan. rahmatullahq@uaar.edu.pk.
Abdul RaufDepartment of Zoology, Faculty of Science, University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
NoshinIlyasDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan.
Qaiser HussainDepartment of Environmental and Soil Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan.
Syed Sabir Hussain ShahDepartment of Botany, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan.
Sabith RehmanDepartment of Botany, Hazara University Mansehra, Mansehra, KPK, Pakistan.
Arshad Mahmood KhanDepartment of Botany, Government Hashmat Ali Islamia Associate College Rawalpindi, Rawalpindi, 46300, Pakistan.
Nasrullah KhanDepartment of Botany, University of Malakand, Chakdara, P.O. Box 18800, Dir Lower, KPK, Pakistan.
Mostafa A Abdel-MaksoudBotany and Microbiology department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Abdul MalikDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Sabiha FatimaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Bushra Hafeez Kianiartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA, 01609, USA.

Funding

King Saud University, KSA RSPD2024R966
6 · The paper itself

Abstract

Medicinal hemp (Cannabis sativa L.) is gaining popularity due to its natural products with potential therapeutic properties, and its cultivation has recently been initiated in developing countries such as Turkey, Iran, India, and Pakistan. The crop is primarily grown using feminized seeds or popular varieties to achieve higher cannabidiol (CBD) yields, with seeds costing approximately $5-10 each. However, limited knowledge exists on hemp seed germination under different seed priming techniques and variations in seedling growth parameters such as root and shoot lengths, as well as the influence of in vitro germination and growth in soil and cocopeat media. This study hypothesizes that various seed priming methods could significantly enhance hemp seed germination and different growth media may distinctly impact seedling growth of the tested cultivars. To test this, the study evaluated four cultivars/chemovars (viz., Fedora-17, Finola, CBD Pink Kush, and a local variety, Gulistan) under five seed priming treatments: indole butyric acid (IBA), moringa leaf extract, KNO3, hydro-priming, and a control (no priming). A randomized complete block design (RCBD) was used, and experimental data (in triplicate) were analyzed through analysis of variance (ANOVA) using a time-to-event approach. The findings revealed that IBA significantly (p < 0.05) improved seed germination rates, with CBD Pink Kush achieving the highest germination response (83%) among the tested cultivars. Furthermore, cocopeat demonstrated superior performance as a seedling growth medium across all cultivars, indicating its potential for hemp cultivation practices. This pioneering study provides a baseline for understanding hemp seed germination and seedling growth under different priming and growth media conditions, offering valuable insights for future research aimed at industrial and medicinal hemp production.

Indexed as

CannabisGerminationSeedlingsSeedsPlant ExtractsPlant ExtractsCannabis sativaGerminationHemp cultivarsSeedling vigorSeed priming

Identifiers

PMID39856131
PMCPMC11759709

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.