Role of Ribosomal Protein bS1 in Orthogonal mRNA Start Codon Selection.

Kristina V Boyko, Rebecca A Bernstein, Minji Kim, Jamie H D Cate

Abstract read

In one paragraph

Article in Biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Rewiring the ribosome to translate proteins encoded in its own RNA.Nature · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kristina V BoykoBiophysics Graduate Group, University of California, Berkeley, California 94720, United States.

Rebecca A BernsteinDepartment of Chemistry, University of California, Berkeley, California 94720, United States.

Minji KimDepartment of Molecular and Cell Biology, University of California, Berkeley, California 94720, United States.

Jamie H D CateDepartment of Chemistry, University of California, Berkeley, California 94720, United States.ORCID 0000-0001-5965-7902

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In many bacteria, the location of the mRNA start codon is determined by a short ribosome binding site sequence that base pairs with the 3'-end of 16S rRNA (rRNA) in the 30S subunit. Many groups have changed these short sequences, termed the Shine-Dalgarno (SD) sequence in the mRNA and the anti-Shine-Dalgarno (ASD) sequence in 16S rRNA, to create "orthogonal" ribosomes to enable the synthesis of orthogonal polymers in the presence of the endogenous translation machinery. However, orthogonal ribosomes are prone to SD-independent translation. Ribosomal protein bS1, which binds to the 30S ribosomal subunit, is thought to promote translation initiation by shuttling the mRNA to the ribosome. Thus, a better understanding of how the SD and bS1 contribute to start codon selection could help efforts to improve the orthogonality of ribosomes. Here, we engineered the

Indexed as

Codon, InitiatorEscherichia coliEscherichia coli ProteinsRibosomal ProteinsRNA, MessengerPeptide Chain Initiation, TranslationalProtein BiosynthesisRibosomesRNA, BacterialRNA, Ribosomal, 16SCodon, InitiatorEscherichia coli ProteinsRibosomal ProteinsRNA, BacterialRNA, MessengerRNA, Ribosomal, 16S

Identifiers

PMID39854700

DOI10.1021/acs.biochem.4c00688

PMCPMC11800381

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.