Evidence map›Paper›PMID 39854619›Full record

ArticleeLife2024

TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders.

Nincy Debeuf, Sahine Lameire, Manon Vanheerswynghels, Julie Deckers, Caroline De Wolf, Wendy Toussaint, Rein Verbeke, Kevin Verstaen, Hamida Hammad, Stijn Vanhee and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nincy Debeuf *Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.ORCID https://orcid.org/0000-0002-4754-4772
Sahine Lameire *Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Manon VanheerswynghelsLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Julie DeckersLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Caroline De WolfLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Wendy ToussaintLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Rein VerbekeLaboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
Kevin VerstaenVIB Single Cell Core, VIB Center, Ghent, Belgium.
Hamida HammadLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Stijn Vanhee *Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Bart N Lambrecht *Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.ORCID https://orcid.org/0000-0003-4376-6834

Funding

Fonds Wetenschappelijk Onderzoek 01M01521Fonds Wetenschappelijk Onderzoek 1244321NFonds Wetenschappelijk Onderzoek 1258921NFonds Wetenschappelijk Onderzoek 1275023NFonds Wetenschappelijk Onderzoek 3G0H1222Fonds Wetenschappelijk Onderzoek G0A7422NiBOF 01M01521
6 · The paper itself

Abstract

Since the precursor frequency of naive T cells is extremely low, investigating the early steps of antigen-specific T cell activation is challenging. To overcome this detection problem, adoptive transfer of a cohort of T cells purified from T cell receptor (TCR) transgenic donors has been extensively used but is not readily available for emerging pathogens. Constructing TCR transgenic mice from T cell hybridomas is a labor-intensive and sometimes erratic process, since the best clones are selected based on antigen-induced CD69 upregulation or IL-2 production in vitro, and TCR chains are polymerase chain reaction (PCR)-cloned into expression vectors. Here, we exploited the rapid advances in single-cell sequencing and TCR repertoire analysis to select the best clones without hybridoma selection, and generated CORSET8 mice (

Indexed as

CD8-Positive T-LymphocytesReceptors, Antigen, T-CellSingle-Cell AnalysisAnimalsClone CellsCOVID-19Lymphocyte ActivationMiceMice, Inbred C57BLMice, TransgenicSARS-CoV-2Spike Glycoprotein, CoronavirusReceptors, Antigen, T-CellSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2immunologyinflammationmouseSARS-CoV-2single-cell RNA analysisTCR transgenic mouseVDJseq

Identifiers

PMID39854619
PMCPMC11684785

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.