Evidence map›Paper›PMID 39854156›Full record

ArticleAnimal models and experimental medicine2025

A novel model of central precocious puberty disease: Paternal MKRN3 gene-modified rabbit.

Bangzhu Chen, Xing Ye, Lihao Chen, Tianping Liu, Guiling Li, Chula Sa, Juan Li, Ke Liu, Weiwang Gu, Gang Wang

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Article in Animal models and experimental medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Bangzhu ChenDepartment of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.ORCID 0000-0003-2526-9551
Xing YeSchool of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
Lihao ChenDepartment of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Tianping LiuGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.
Guiling LiGuangdong Medical Laboratory Animal Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.
Chula SaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.
Juan LiGuangdong Medical Laboratory Animal Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.
Ke LiuGuangdong Medical Laboratory Animal Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.
Weiwang GuGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China.
Gang WangGuangdong Medical Laboratory Animal Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.

Funding

Guangdong Medical Science and Technology Research Fund Project, China B2024069Guangzhou Science and Technology Plan Project, China 2024A04J4923Guangzhou Science and Technology Plan Project, China SL2023A04J02229National Natural Science Foundation of China 82101937
6 · The paper itself

Abstract

backgroundMakorin ring finger protein 3 gene (MKRN3) gene mutation is the most common genetic cause of central precocious puberty (CPP) in children. Due to the lack of ideal MKRN3-modified animal model (MKRN3-modified mice enter puberty only 4-5 days earlier than normal mice), the related research is limited.

methodsTherefore, the MKRN3-modified rabbit was developed using CRISPR (clustered regularly interspaced short palindromic repeats) gene editing technology. The genotype identification and phenotype evaluation of MKRN3-modified rabbits were carried out.

resultsThe first estrus of MKRN3-modified female rabbits was observed ~27 days earlier than that of wild-type female rabbits, with a typical CPP phenotype. This study found increased gonadotropin releasing hormone (GnRH) and decreased gonadotropin inhibiting hormone (GnIH) in the hypothalamus of the CPP rabbit model with MKRN3 gene mutation. Although this study failed to fully clarify the pathogenesis of CPP caused by MKRN3 mutation, it found some differentially expressed genes and potential pathways through transcriptome sequencing.

conclusionsThis study established a novel CPP model: paternal MKRN3 gene-modified rabbit. It is hoped that the establishment of this model will help researchers better understand, treat, and prevent CPP in the future.

Indexed as

Disease Models, AnimalPuberty, PrecociousRibonucleoproteinsUbiquitin-Protein LigasesAnimalsAnimals, Genetically ModifiedFemaleGene EditingGonadotropin-Releasing HormoneHypothalamusMaleMutationRabbitsGonadotropin-Releasing HormoneRibonucleoproteinsUbiquitin-Protein Ligasescentral precocious pubertygonadotropin inhibiting hormone (GnIH)gonadotropin releasing hormone (GnRH)MKRN3rabbit

Identifiers

PMID39854156
PMCPMC11904109

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.