ArticleVeterinary medicine and science2025
Rapid and Environment-Friendly LC-MS/MS for Simultaneous Analysis of Amino Acids in Veterinary Medicine.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundAmino acid supplements are crucial for animal health and productivity. Traditional analysis methods face limitations like complexity, long testing times and toxic reagents. Therefore, a more efficient and reliable method is needed.
objectivesThis study aimed to develop and validate an efficient method for the simultaneous analysis of eight amino acids commonly used in veterinary medicine: alanine, arginine, glutathione, lysine, ornithine, methionine, threonine and tryptophan.
methodsWe analysed eight veterinary amino acid preparations. From 100 registered products, we selected 35. After confirming ingredients, we diluted them to 1 mg/L with 50% acetonitrile (ACN) and filtered them using a 0.2 µm RC filter for liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis.
resultsAll analytes showed excellent linearity (r
conclusionsThe method developed in this study offers an efficient and reliable approach for the simultaneous analysis of essential amino acids in veterinary medicine. Implementing this method can improve the quality control of amino acid products, enhancing animal health and productivity. This study also highlights the need for stringent domestic management and continuous monitoring. By overcoming traditional technique limitations, this validated method ensures the quality and efficacy of amino acid supplements in the veterinary industry.
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