Evidence map›Paper›PMID 39852905›Full record

ArticleVeterinary sciences2025

Effect of Bovine Serum Albumin (BSA) Concentration on Cryopreservation of Booroolong Frog Sperm with Evaluation of Post-Thaw Motility in Caffeine.

Zara M Anastas, Aimee J Silla, Phillip G Byrne, Rebecca J Hobbs, Michael S McFadden, Jonathan Daly, Justine K O'Brien

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zara M AnastasEnvironmental Futures, School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.ORCID 0009-0004-0046-4048
Aimee J SillaEnvironmental Futures, School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.ORCID 0000-0002-2277-9030
Phillip G ByrneEnvironmental Futures, School of Earth, Atmospheric and Life Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
Rebecca J HobbsTaronga Institute of Science and Learning, Taronga Conservation Society Australia, Mosman, NSW 2088, Australia.ORCID 0000-0001-6989-2447
Michael S McFaddenTaronga Institute of Science and Learning, Taronga Conservation Society Australia, Mosman, NSW 2088, Australia.ORCID 0000-0001-6811-8505
Jonathan DalyTaronga Institute of Science and Learning, Taronga Conservation Society Australia, Mosman, NSW 2088, Australia.
Justine K O'BrienTaronga Institute of Science and Learning, Taronga Conservation Society Australia, Mosman, NSW 2088, Australia.ORCID 0000-0003-2011-9626

Funding

Australian Research Council DE210100812Ecological Society of Australia Holsworth Wildlife Research EndowmentZoo and Aquarium Association Wildlife Conservation Fund
6 · The paper itself

Abstract

Reproductive technologies, including sperm cryopreservation, offer conservationists enhanced capacity to genetically manage populations and improve the outcomes of conservation breeding programs (CBPs). Despite this potential, the post-thaw quality of amphibian sperm is highly variable following cryopreservation, and research focused on protocol refinement is needed. The aim of this study was twofold: (1) to investigate the effect of the addition of bovine serum albumin (BSA) to the cryopreservation medium (pre-freeze), and (2) the effect of the addition of caffeine to the activation medium (post-thaw), on post-thaw sperm characteristics in the critically endangered Booroolong frog (

Indexed as

amphibianbiobankingbiorepositorybovine serum albuminbreeding programcaffeineconservationreproductive technologiessperm cryopreservationspermic urine

Identifiers

PMID39852905
PMCPMC11769386

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.