ArticleMarine drugs2025
Expression Analysis of Heavy-Chain-Only Antibodies in Cloudy Catshark and Japanese Bullhead Shark.
Reo Uemura, Susumu Tanimura, Nao Yamaguchi, Ryuichi Kuroiwa, Gabriel Takashi Andrés Tsutsumi, Toshiaki Fujikawa, Kiyoshi Soyano, Kohsuke Takeda, Yoshimasa Tanaka
Abstract read
In one paragraphArticle in Marine drugs, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
9 authors.
Reo UemuraDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
Susumu TanimuraDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.ORCID 0000-0002-4336-4934 Nao YamaguchiDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
Ryuichi KuroiwaDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
Gabriel Takashi Andrés TsutsumiDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.
Toshiaki FujikawaInstitute for East China Sea Research, Organization for Marine Science and Technology, Nagasaki University, 1551-7, Taira-machi, Nagasaki 851-2213, Japan.
Kiyoshi SoyanoInstitute for East China Sea Research, Organization for Marine Science and Technology, Nagasaki University, 1551-7, Taira-machi, Nagasaki 851-2213, Japan.
Kohsuke TakedaDepartment of Cell Regulation, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.ORCID 0000-0002-8359-8399 Yoshimasa TanakaCenter for Medical Innovation, Nagasaki University, Sakamoto 1-7-1, Nagasaki 852-8588, Japan.ORCID 0000-0002-5024-0614 Funding
Japan Agency for Medical Research and Development 23-A-19Japan Agency for Medical Research and Development 24ama121032j0003Japan Agency for Medical Research and Development 24fk0310518h0003Japan Agency for Medical Research and Development A48Japan Agency for Medical Research and Development A90Japan Agency for Medical Research and Development JP223fa627004Japan Science and Technology Agency JPMJST2281Ministry of Education, Culture, Sports, Science and Technology JP16K08844Ministry of Education, Culture, Sports, Science and Technology JP23H02898Ministry of Education, Culture, Sports, Science and Technology JP23K06117Ministry of Education, Culture, Sports, Science and Technology JP23K06677Research Funding granted by Nagasaki University President Shigeru Kohno YTSHIONOGI INFECTIOUS DISEASE RESEARCH PROMOTION FOUNDATION 2023houga
6 · The paper itselfAbstract
Heavy chain-only antibodies in sharks are called immunoglobulin new antigen receptors (IgNAR), consisting of one variable region (VNAR) and five constant regions (C1-C5). The variable region of IgNAR can be expressed as a monomer composed of a single domain, which has antigen specificity and is thus gaining attention as a next-generation antibody drug modality. In this study, we analyzed IgNAR of the cloudy catshark and Japanese bullhead shark, small demersal sharks available in the coastal waters of Japan. By analyzing the IgNAR gene sequence and comparing it with the constant regions of five other known shark species, high homology was observed in the C4 region. Consequently, we expressed the recombinant protein of the C4 domain from the cloudy catshark in
Indexed as
Immunoglobulin Heavy ChainsReceptors, AntigenSharksAmino Acid SequenceAnimalsFish ProteinsJapanRatsRecombinant ProteinsFish ProteinsImmunoglobulin Heavy ChainsReceptors, AntigenRecombinant Proteinscloudy catsharkheavy-chain-only antibodyimmunoglobulin new antigen receptorJapanese bullhead sharkVNAR-domain antibody
Identifiers
PMID39852530
PMCPMC11767079
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