Evidence map›Paper›PMID 39852250›Full record

ArticleMembranes2025

A STEDable BF

Anaïs C Bourgès, Massimiliano Garre, Dan Wu, Donal F O'Shea

Abstract read
In one paragraph

Article in Membranes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anaïs C BourgèsDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen's Green, D02 YN77 Dublin, Ireland.ORCID 0000-0002-1789-3816
Massimiliano GarreDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen's Green, D02 YN77 Dublin, Ireland.ORCID 0000-0001-9332-0270
Dan WuDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen's Green, D02 YN77 Dublin, Ireland.ORCID 0000-0002-5316-3706
Donal F O'SheaDepartment of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen's Green, D02 YN77 Dublin, Ireland.

Funding

Enterprise Ireland DTIFScience Foundation Ireland 12/RC/2275_P2Science Foundation Ireland 18/RI/5723
6 · The paper itself

Abstract

The endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear membrane-endoplasmic reticulum (NM-ER) staining fluorophore with emission maxima at 650 nm. NM-ER is compatible with fixed and live cell imaging and stimulated emission depletion microscopy (STED) showing significant improvement in resolution when compared to comparable confocal laser scanning microscopy. The imaging versatility of NM-ER was illustrated through its compatible use with other fluorophores for co-imaging with DNA, nuclear pores and lamina allowing cellular abnormalities to be identified. NM-ER alone, or in use with other nuclear region labels could be an important tool for the investigation of nuclear transport and associated cellular processes.

Indexed as

BF2-azadipyrrometheneDNAendoplasmic reticulumfluorophoresnuclear laminanuclear membranenuclear poresSTED microscopysuper resolution imaging

Identifiers

PMID39852250
PMCPMC11766805

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.