The Research Progress of Single-Molecule Sequencing and Its Significance in Nucleic Acid Metrology.

Yajun Wang, Jingjing Liu, Zhendong Wang, Mei Zhang, Yongzhuo Zhang

Abstract readReview

In one paragraph

Review in Biosensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yajun WangCollege of Information and Control Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.

Jingjing LiuCollege of Information and Control Engineering, China Northeast Electric Power University, Jilin 132011, China.ORCID 0000-0002-9602-8918

Zhendong WangCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.

Mei ZhangCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.

Yongzhuo ZhangCenter for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.

Funding

National Key R&D Program of China 2021YFC2800400Programme and State Administration for Market Regulation Technology Program Project 2022MK177Research on key techniques of whole genome sequencing measurement AKYZD2202-1

6 · The paper itself

Abstract

Single-molecule sequencing technology, a novel method for gene sequencing, utilizes nano-sized materials to detect electrical and fluorescent signals. Compared to traditional Sanger sequencing and next-generation sequencing technologies, it offers significant advantages, including ultra-long read lengths, rapid sequencing, and the absence of amplification steps, making it widely applicable across various fields. By examining the development and components of single-molecule sequencing technology, it becomes clear that its unique characteristics provide new opportunities for advancing metrological traceability. Notably, its direct detection capabilities offer a novel approach to nucleic acid metrology. This paper provides a detailed overview of library construction, signal generation and detection, and data analysis methods in single-molecule sequencing and discusses its implications for nucleic acid metrology.

Indexed as

Nucleic AcidsSequence Analysis, DNABiosensing TechniquesHigh-Throughput Nucleotide SequencingHumansNucleic Acidsnanoporenucleic acid metrological traceabilitysingle-molecule sequencing

Identifiers

PMID39852055

DOI10.3390/bios15010004

PMCPMC11763189

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.