Evidence map›Paper›PMID 39851549›Full record

ArticleCells2025

Discovery of Evolutionary Loss of the Ubiquitin-like Autophagy-Related ATG12 System in a Lineage of Apicomplexa.

Xiaoxia X Lin, Yun D Bai, Sichang T Wang, Akira Nozawa, Tatsuya Sawasaki, Tatsunori Masatani, Kenji Hikosaka, Masahito Asada, Hirokazu Sakamoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiaoxia X LinDepartment of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan.
Yun D BaiDepartment of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan.
Sichang T WangDepartment of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan.
Akira NozawaProteo-Science Center (PROS), Ehime University, Matsuyama 790-0825, Japan.
Tatsuya SawasakiProteo-Science Center (PROS), Ehime University, Matsuyama 790-0825, Japan.
Tatsunori MasataniLaboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.ORCID 0000-0002-2347-4079
Kenji HikosakaDepartment of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan.
Masahito AsadaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-0834, Japan.ORCID 0000-0003-0042-6856
Hirokazu SakamotoDepartment of Infection and Host Defense, Graduate School of Medicine, Chiba University, Chiba 263-8522, Japan.ORCID 0000-0001-9368-105X

Funding

Japan Agency for Medical Research and Development JP23ama121010j0002Japan Agency for Medical Research and Development JP23fk0310521h0002Japan Society for the Promotion of Science JP19K16627Japan Society for the Promotion of Science JP21KK0285Japan Society for the Promotion of Science JP22H04633Japan Society for the Promotion of Science JP23K18008
6 · The paper itself

Abstract

The autophagy-related ubiquitin-like conjugation systems, the ATG8 and ATG12 systems, are universally conserved in eukaryotes. However, the covalent bond in the ATG12 system has recently been shown to be evolutionarily lost in Apicomplexa. Here, we show that all genes associated with the ATG12 system are absent in piroplasmida, a lineage within Apicomplexa. Comparative genomics of ATGs further shows that piroplasm ATG3 has lost the region necessary for ATG12 binding. However, our in vitro functional analysis using recombinant proteins demonstrated that ATG3 retained the ability to interact with ATG8 in

Indexed as

ApicomplexaAutophagyAutophagy-Related Protein 12Evolution, MolecularUbiquitinAutophagy-Related Protein 8 FamilyAutophagy-Related ProteinsPhylogenyProtozoan ProteinsAutophagy-Related Protein 12Autophagy-Related Protein 8 FamilyAutophagy-Related ProteinsProtozoan ProteinsUbiquitinApicomplexaATG12 systemautophagypiroplasmida

Identifiers

PMID39851549
PMCPMC11764061

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.