Metagenomic Insights into Pollutants in Biorefinery and Dairy Wastewater: rDNA Dominance and Electricity Generation in Double Chamber Microbial Fuel Cells.

Khaya Pearlman Shabangu, Manimagalay Chetty, Babatunde Femi Bakare

Abstract read

In one paragraph

Article in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Isolation and assessment of fungi derived from wastewater for sustainable energy generation.Folia microbiologica · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Khaya Pearlman ShabanguGreen Engineering & Sustainability Research Group, Department of Chemical Engineering, Faculty of Engineering and the Built Environment, Durban University of Technology, Steve Biko Campus, S3 L3, P.O. Box 1334, Durban 4000, South Africa.ORCID 0000-0002-2989-3792

Manimagalay ChettyGreen Engineering & Sustainability Research Group, Department of Chemical Engineering, Faculty of Engineering and the Built Environment, Durban University of Technology, Steve Biko Campus, S3 L3, P.O. Box 1334, Durban 4000, South Africa.ORCID 0000-0003-4035-739X

Babatunde Femi BakareEnvironmental Pollution and Remediation Research Group, Department of Chemical Engineering, Mangosuthu University of Technology, P.O. Box 12363, Jacobs 4062, South Africa.

Funding

Cape Peninsula University of Technology (CPUT) 3CSRD VA 1Durban University of Technology (DUT) 2

6 · The paper itself

Abstract

This study evaluates the potential of biorefinery and dairy wastewater as substrates for electricity generation in double chamber Microbial Fuel Cells (DCMFC), focusing on their microbial taxonomy and electrochemical viability. Taxonomic analysis using 16S/18S rDNA-targeted DGGE and high-throughput sequencing identified Proteobacteria as dominant in biorefinery biomass, followed by Firmicutes and Bacteriodota. In dairy biomass, Lactobacillus (77.36%) and Clostridium (15.70%) were most prevalent. Biorefinery wastewater exhibited the highest bioelectrochemical viability due to its superior electrical conductivity and salinity, achieving a voltage yield of 65 mV, compared to 75.2 mV from mixed substrates and 1.7 mV from dairy wastewater. Elevated phosphate levels in dairy wastewater inhibited bioelectrochemical processes. This study recommends Biorefinery wastewater as the most suitable purely organic substrate for efficient bioelectricity generation and scaling up of MFCs, emphasising the importance of substrate selection for optimal energy output for practical and commercial viability.

Indexed as

Bacteriodotaelectrical conductivityFirmicutesMicrobial Fuel CellProteobacteriasalinitywastewater

Identifiers

PMID39851362

DOI10.3390/bioengineering12010088

PMCPMC11761944

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.