ArticleMethodsX2025
A dual expression plasmid with Microcin B17 compatible with both prokaryotic and mammalian systems.
Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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5 authors.
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Abstract
Proteic plasmid addiction systems, such as the control of cell death (Ccd), have been used for efficient plasmid DNA recombination. The CcdB toxin, which has a relatively long sequence of 309 bp, has been the predominant choice for this purpose. However, the need for shorter peptide toxins has emerged. In this study, we evaluated the utility of microcin B17 (MccB17), a peptide consisting of 43 amino acids, in promoting DNA recombination within pgMAX-II, a dual expression plasmid for both prokaryotic and mammalian systems. The insertion of the α-peptide gene from lacZ (α-complementation) demonstrated highly efficient cloning of external DNA in the pgMAX-II/MccB17 plasmid. In both
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