Evidence map›Paper›PMID 39849603›Full record

ArticleStem cell research & therapy2025

Functional differentiation of human dental pulp stem cells into neuron-like cells exhibiting electrophysiological activity.

B Pardo-Rodríguez, A M Baraibar, I Manero-Roig, J Luzuriaga, J Salvador-Moya, Y Polo, R Basanta-Torres, F Unda, S Mato, Gaskon Ibarretxe and 1 more

Abstract read
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In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

B Pardo-RodríguezDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
A M BaraibarDepartment of Neurosciences, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
I Manero-RoigDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
J LuzuriagaDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
J Salvador-MoyaDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
Y PoloPolimerbio SL, Donostia-San Sebastián, 20014, Spain.
R Basanta-TorresDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
F UndaDepartment of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
S MatoDepartment of Neurosciences, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
Gaskon Ibarretxe *Department of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain. gaskon.ibarretxe@ehu.eus.ORCID https://orcid.org/0000-0002-4961-7402
Jose Ramon Pineda *Department of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain. joseramon.pinedam@ehu.eus.ORCID https://orcid.org/0000-0002-0900-7466

Funding

ARSEP Foundation ARSEP-1310Euskal Herriko Unibertsitatea COLAB22/07Euskal Herriko Unibertsitatea PhD fellowship PIFBUR21/05Eusko Jaurlaritza Bikaintek PostDoc grant 010-B1/2023Eusko Jaurlaritza ELKARTEK program MYOZET KK-2024/00111Eusko Jaurlaritza IT1473-22Eusko Jaurlaritza IT1751-22Eusko Jaurlaritza PhD fellowship PRE_2023_2_0038Eusko Jaurlaritza PhD fellowship PRE_2023_2_0112Eusko Jaurlaritza PIBA_2023_1_0046Eusko Jaurlaritza PostDoc grant POS_2019_1_0041Instituto de Salud Carlos III PI21/00629Ministerio de Ciencia e Innovación PID2019-104766RB-C21Ministerio de Ciencia e Innovación PID2023-152704OB-I00Osasun Saila, Eusko Jaurlaritzako "Strengthening strategic health research" program No. 2023111031Osasun Saila, Eusko Jaurlaritzako "Strengthening strategic health research" program No. 2023333035POLIMERBIO S.L. contract 2023.0012
6 · The paper itself

Abstract

BACKGROUND AND

aimHuman dental pulp stem cells (hDPSCs) constitute a promising alternative for central nervous system (CNS) cell therapy. Unlike other human stem cells, hDPSCs can be differentiated, without genetic modification, to neural cells that secrete neuroprotective factors. However, a better understanding of their real capacity to give rise to functional neurons and integrate into synaptic networks is still needed. For that, ex vivo differentiation protocols must be refined, especially to avoid the use of fetal animal serum. The aim of our study is to improve existing differentiation protocols of hDPSCs into neuron-like cells.

methodsWe compared the effects of the (1) absence or presence of fetal serum during the initial expansion phase as a step prior to switching cultures to neurodifferentiation media. We (2) improved hDPSC neurodifferentiation by adding retinoic acid (RA) and potassium chloride (KCl) pulses for 21 or 60 days and characterized the results by immunofluorescence, digital morphometric analysis, RT-qPCR and electrophysiology.

resultsWe found that neural markers like Nestin, GFAP, S100β and p75

conclusionOur study demonstrates that hDPSCs can be differentiated to neuronal-like cells that display functional excitability and thus evidence the potential of these easily accessible human stem cells for nerve tissue engineering. These results highlight the importance of choosing an appropriate culture protocol to successfully neurodifferentiate hDPSCs.

Indexed as

Cell DifferentiationDental PulpNeuronsStem CellsCells, CulturedDoublecortin ProteinHumansNerve Tissue ProteinsTretinoinDCX protein, humanDoublecortin ProteinNerve Tissue ProteinsTretinoinAction potentialGABAHuman dental pulp stem cellsNeurodifferentiationSerum-freeSynapsinWhole-cell patch clamp

Identifiers

PMID39849603
PMCPMC11756023

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