Integrating Retinal Segmentation Metrics with Machine Learning for Predictions from Mouse SD-OCT Scans.

purposeThis study aimed to initially test whether machine learning approaches could categorically predict two simple biological features, mouse age and mouse species, using the retinal segmentation metrics.

methodsThe retinal layer thickness data obtained from C57BL/6 and DBA/2J mice were processed for machine learning after segmenting mouse retinal SD-OCT scans. Twenty-two models were trained to predict the mouse groups. The best neural network model was optimized for better outcomes. Prediction accuracy, the area under the curve, sensitivity, specificity, precision, and F-1 score values were obtained.

resultsThe Wilcoxon Signed-Rank test provided significantly higher validation accuracy for neural networks than decision trees, discriminant analysis, support vector machines, and k-nearest neighbor classifiers (

conclusionsThese findings validate that machine learning, using segmentation metrics instead of images, can effectively analyze retinal OCT scans in mice for categorical predictions in experimental models. Expanding this approach with additional features, including histopathological and functional correlations, is expected to improve the prediction power further, promising valuable applications to predict more complex outcomes in experimental and clinical studies.