From water to land-Usage of Generalized Unified Threshold models of Survival (GUTS) in an above-ground terrestrial context exemplified by honeybee survival data.

In regulatory aquatic risk assessment, toxicokinetic-toxicodynamic (TKTD) methods, such as the generalized unified threshold model of survival (GUTS), are already established and considered ready for use, whereas TKTD methods for aboveground terrestrial species, like arthropods, are less developed and currently not intended for risk assessment. This could be due to the fact that exposure in aboveground terrestrial systems is more event-based (feeding, contact, overspray, etc.), whereas exposure in aquatic systems is simply related to substance concentrations in the surrounding water. To provide a generic TKTD framework for terrestrial invertebrates, we propose a new GUTS variant that includes an intermediate buffer between the external exposure and inside of the organism. This buffer can be interpreted as residues on the exoskeleton or in the stomach, depending on the uptake route. Such an uptake behavior is mechanistically reasonable and observable in laboratory experiments. This GUTS variant, BufferGUTS, is particularly suitable for discrete or discretized exposure scenarios. Testing our model on honeybee datasets for 13 pesticides reveals a similar or better reproduction of survival curves than existing models (GUTS-RED and BeeGUTS) while keeping the number of parameters the same and making no substance or species-specific assumptions. The proposed new BufferGUTS approach can prospectively be used to derive TKTD parameters for a variety of terrestrial arthropod species. A standardized model definition for terrestrial species will facilitate the comparison and extrapolation of parameters between species and the applicability for terrestrial risk assessments.